SummaryA simple, rapid and accurate, routine-HPLC method is described for simultaneous determination of acetaminophen, caffeine and chlorpheniramine maleate in a new tablet formulation. Chromatographic separation of the three pharmaceuticals was achieved on a Hypersil CN column (150 x 5.0 mm, 5 Ixm) using a mobile phase comprising a mixture of acetonitrile, an ion-pair solution and tetrahyclrofuran (13:14:8Z v/v, pH 4.5). The flow-rate was changed from 1.0 mL min 1 (in 0 ~ Z5 min) to 1.8 mL min 1 (after 3.5 min). Detection was 223 nm. Separation was complete in < 10 min. The method was validated for system suitability, hnearity, accuracy, precision, limits of detection and quantitation, and robustness. Linearity, accuracy and precision were found to be acceptable over the ranges 31.6 -315.8 Ixg mL 1 for acetaminophen, 9.5 -94.6 I~g mL 1 forcaffeine and 1.4-13.8 I~g mL 1 forchlorpheniramine maleate.

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