Seasonal Influenza H3N2 virus poses a great threat to public health, but its vaccine efficacy remains suboptimal. One critical step in influenza vaccine production is the viral passage in embryonated eggs. Recently, the strength of egg passage adaptation was found to be rapidly increasing with time driven by convergent evolution at a set of functionally important codons in the hemagglutinin (HA1). In this study, we aim to take advantage of the negative correlation between egg passage adaptation and vaccine effectiveness (VE) and develop a computational tool for selecting the best candidate vaccine virus (CVV) for vaccine production. Using a probabilistic approach known as mutational mapping, we characterized the pattern of sequence evolution driven by egg passage adaptation and developed a new metric known as the adaptive distance (AD) which measures the overall strength of egg passage adaptation. We found that AD is negatively correlated with the influenza H3N2 vaccine effectiveness (VE) and ~75% of the variability in VE can be explained by AD. Based on these findings, we developed a computational package that can Measure the Adaptive Distance and predict vaccine Effectiveness (MADE). MADE provides a powerful tool for the community to calibrate the effect of egg passage adaptation and select more reliable strains with minimum egg-passaged changes as the seasonal A/H3N2 influenza vaccine.

Enhanced equalization phase noise (EEPN), generated from the uncompensated dispersion experienced by laser phase noises, can cause serious damage to the transmission quality of optical fiber systems. In this work, the performance of a wideband Nyquist-spaced long-haul nonlinear optical fiber communication systems suffering from EEPN is investigated and discussed through split-step numerical simulations and analytical models based on the perturbation analysis, in the cases of digital nonlinearity compensation (NLC) and electronic dispersion compensation (EDC). The efficiency and the accuracy of the analytical models were validated via simulations, considering the different symbol rates and modulation formats. The performance of the C-band transmission was comprehensively studied based on the model. Our results reveal that the growth of symbol rates and transmission distances aggravates the distortions in the C-band system.