MicroRNA-215 (miR-215) has previously been demonstrated to be dysregulated in a number of human malignancies and to be correlated with tumor progression. However, the expression and function of miR-215 in non-small cell lung cancer (NSCLC) has remained to be elucidated. Therefore, the present study aimed to investigate the effects of miR-215 in NSCLC tumorigenesis and development. Reverse transcription-quantitative polymerase chain reaction was used to evaluate miR-215 expression in NSCLC cell lines and primary tumor tissues. The association between miR-215 expression and certain clinicopathological factors was also determined, and the effects of miR-215 on the biological behavior of NSCLC cells were investigated. In addition, the potential regulatory function of miR-215 on zinc finger E-box-binding homeobox 2 (ZEB2) expression was examined. miR-215 expression was significantly downregulated in NSCLC cell lines and clinical specimens. Reduced miR-215 expression was significantly associated with lymph node metastasis and advanced TNM stage. Overexpression of miR-215 inhibited NSCLC cell proliferation, invasion and migration, and promoted cell apoptosis in vitro, and suppressed tumorigenicity in vivo. Furthermore, luciferase reporter assay analysis identified ZEB2 as a direct target of miR-215. These findings indicated that miR-215 may act as a tumor suppressor in NSCLC and may serve as a novel therapeutic agent for miR-based therapy.

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Background: At the beginning of the outbreak of coronavirus infected disease 2019 (COVID-19), children in Wuhan were experiencing an extremely strong influenza A epidemic. This study is aimed to explore the epidemic dynamics characteristics of children with influenza A and its correlation with the early stage spread of COVID-19 in Wuhan.Methods: This is a retrospective single-center clinical study.From November 28, 2019 to January 23, 2020, a total of 7904 outpatient children with signs of respiratory tract infection were admitted, and a total of 10102 throat swabs were collected. All the detection were performed to the throat swabs of patients, which include the epidemic statues, detection rate, duration of Flu A and B persistence in airway, and the positive rate of COVID-19 nucleic acid.Results: A total of 10102 throat swabs were obtained from children with respiratory symptoms, including 5450 (53.9%) male and 4652 (46.1%) female. 2899 (28.7%) cases were positive for Influenza A. There were 617 (6.1% ) cases of Influenza B . In group of Influenza A , the lowest positive rate was in the infants less than 1 year old (18.4%), and the highest in the group 12 year old (32.1%). During the period of high prevalence of influenza A, there was a low level of infection of influenza B. The detection rate of each age group fluctuated from 3% to 10%. 73.7% of children's influenza A and B virus turned negative within 7 days, and very few children's respiratory influenza virus can last even more than 1 month. Among 35 throat swabs detected with qRT-PCR, 11 (31.4%) were positive for Flu A, and all children were negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).Conclusions: In the early stage of SARS-CoV-2 transmission, children in Wuhan were experiencing a high intensity of influenza A pandemic, and there was no the mixed infection of SARS-CoV-2 with influenza A. COVID-19 spread caught up the tail of influenza A pandemic.