SummaryPolyacrylamide gel filled capillaries for electrophoresis can be improved in performance and stability by a specially optimized and reproducible production procedure. After surface pretreatment as described in this contribution, gels polymerized in capillaries are stable for long series of routine separations. Bubbles formation during gel polymerization can be avoided. The procedure is compared with those described by other authors. Separations of different oligonucleotide samples illustrate the quality of the gel capillaries obtained. The peak symmetry achieved proved to be better than that obtained without surface pretreatment.

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Short Communications the lactone homologs) also increase at conditions forcing low elution temperatures 1101, but for the resolution of the lactone enantiomers, the effect is stronger than for all other cases investigated Enantioselective resolution of y-lactones on the alkyl-acetylcc-cyclodextrin/0V-1701-OH stationary phase used requires unusual chromatographic conditions: Low elution temperatures are more important than conditions fitting van Deemter curves and optimized TZ values of homologs. For the 25 m x 0.23 mm i.d.column, a program rate of 1.5"/min is suitable and carrier gas flow rates somewhat exceeding the optimum (50 cm/s for the nonalactones and 1 m/s for the dodecalactones).The example once more demonstrated to us that optimum separation may be obtained under conditions far removed from those derived from "theory", and that experimental optimization of gas velocities and temperature program rates, including rather extreme conditions, is important.