Kepik Hijau (Nezara viridula L.) merupakan hama penting bagi beberapa tanaman pangan seperti kedelai,  padi  dan  jagung.  Salah  satu  upaya  untuk  mengendalikan  hama  adalah  dengan pemanfaatan  entomopatogen,  seperti  virus,  bakteri  maupun  jamur.  Salah  satu  jamur entomopatogen  yang berpotensi untuk dieksploitasi adalah Beauveria bassiana yang mempunyai berbagai jenis inang. Belum diketahui patogenesitas B. bassiana yang diisolasi dari beberapa jenis inang terhadap hama kepik hijau (N. viridula), untuk itulah penelitian ini dilakukan dengan tujuan untuk mengetahui patogenesitas B. bassiana yang diisolasi dari   inang yang berbeda terhadap hama kepik hijau (N. viridula). Penelitian ini dilakukan di Laboratorium Agens Hayati (LAH) Kalasey, Balai Perlindungan dan Pengujian Mutu Tanaman Pangan dan Hortikultura (BPPMTPH), Dinas Pertanian dan Peternakan Daerah Provinsi Sulawesi Utara.  Penelitian dilakukan secara in- vitro dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan yaitu (Kontrol negatif , N1: suspensi B. bassiana isolat WBC (wereng batang coklat), N2: suspensi B. bassiana isolat Jati Sari sebagai kontrol positif (rekomendasi Kementan), N3: suspensi B. bassiana isolat Paraeucosmetus pallicornis (kepik hitam), N4: suspensi B. bassiana isolat Leptocorisa acuta (walang sangit) dan N5: suspensi B. bassiana isolat Scotinopara coarctata (kepinding tanah). Perlakuan menggunakan konsentrasi atau kerapatan konidia 109/ml air, dengan 5 ulangan. Data dianalisis dengan Uji Anava 95% dan analisis probit untuk menentukan LT50  (Lethal Time 50). Hasil penelitian menunjukkan bahwa jamur B. bassiana isolat Jati Sari memiliki patogenisitas tertinggi dengan membunuh kepik hijau (N. viridula) sampai 100% pada hari ke-5 HSP (hari setelah perlakuan), sedangkan isolat lain semuanya pada hari ke-6 HSP. Nilai  LT50  (waktu yang dibutuhkan untuk membunuh 50% serangga uji) terdapat pada isolat P. pallicornis yaitu  2,1 hari. Persentasi mortalitas pada hari ke-3 HSP tertinggi terdapat pada perlakuan isolat P. pallicornis dengan rata-rata mortalitas sebesar 78% diikuti isolat L. acuta dan S. coarctata masing-masing sebesar 68%   dan isolat Jati sari sebesar 58%, sedangkan mortalitas terendah terdapat pada perlakuan isolat WBC yaitu sebesar 32%. Tidak ada perbedaan patogenisitas yang signifikan diantara isolat Jati Sari, P. pallicornis, L. acuta maupun S. coarctata kecuali isolat WBC.Kata kunci: Beauveria bassiana; Entomopatogen; Nezara viridula Pathogenicity of Beauveria bassiana (Bals.) Viull. Isolated from Several Host Species Against Green Ladybug, Nezara viridula L. (Hemiptera: Pentatomidae) ABSTRACTGreen Ladybugs (Nezara viridula L.) are important pests for several food crops such as soybeans, rice and corn. One of the efforts to control this pest is the use of entomopathogens, such as viruses, bacteria and fungi. One of the entomopathogenic fungi that has the potential to be exploited is the fungus Beauveria bassiana. The fungi B. bassiana has various of hosts. There are no data on the pathogenicity  of  B.  bassiana  isolated  from  several  host  species  against  green  ladybugs  (Nviridula), for that reason this study was conducted with the aim of knowing the pathogenicity of B. bassiana isolated from different hosts against green ladybugs (N. viridula).. This research was conducted at Laboratory of Biological Control Agents, the Center for the Protection and Quality Testing of Food Crops and Horticulture, the Regional Agriculture and Livestock Service Office of North Sulawesi Province. The research was conducted in-vitro using a completely randomized design (CRD) with 6 treatments, namely (Negative control, N1: suspension B. bassiana, WBC isolate (brown planthopper), N2: B. bassiana suspension, Jati Sari isolate as a positive control (Ministry of Agriculture Recommendation), N3 : B. bassiana suspension isolate Paraeucosmetus pallicornis (black ladybug), N4: suspension B. bassiana isolate Leptocorisa acuta (Rice ear bug) and N5: suspension B. bassiana isolate Scotinopara coarctata (rice black bug) Treatment using conidia concentration or density 109  / ml water, with 5 replications. Data were analyzed with Anava test 95% and probit analysis to determine LT50 (Lethal Time 50). The results showed that the fungus B. bassiana Jati Sari isolate had the highest pathogenicity, because this isolate was able to kill green ladybugs (N viridula) to 100% on the 5th day of DAT (the day after treatment), while the other isolates were all on the 6th DAT. While the value of LT50  (time needed to kill 50% of the insect test) was found in P. pallicornis isolates which were 2,1 days of DAT. Because LT50 in all isolate treatments was between the 2nd and 3rd day of DAT, the Anava test for mortality was carried out on the 3rd day of DAT. The highest percentage of mortality on the 3rd day of DAT was found in the treatment of P. pallicornis isolates with an average mortality of 78% followed by L. acuta and S. coarctata isolates each at 68% and Jati sari isolates at 58%, while the lowest mortality was found in the treatment of WBC isolates, namely 32%. There was no significant difference in pathogenicity between Jati Sari, P. pallicornis, L. acuta and S. coarctata isolates except for WBC isolates.Keywords: Beauveria bassiana; Entomopatogen;  Nezara viridula

Brown planthoppers have been reported to be resistant to various types of insecticides. One of the environmentally friendly controls is the use of entomopathogenic fungi. This study aims to test the effectiveness of the entomopathogenic fungus M. huainamdangense local isolates against brown planthoppers. Pathogenicity testing of local isolates of M. huainamdangense used a completely randomized design (CRD) with five treatments, namely K1 (Density 106), K2 (Density 107), K3 (Density 108), K4 (Density 109) and K0 as control. Each treatment was repeated 5 (five) times. The results showed that the fastest treatment to kill insects up to 100% was in the K4 treatment (density 109). The results of the probit analysis showed the LT50 value in the conidium density treatment 109 (K4) at 1.6 days. Meanwhile, the LC50 value showed a concentration of 108.9 conidium/ml on the second day. The use of a lower concentration of conidia density was able to kill 50% of insects in a longer time. Keywords: Metarhizium huainamdangense; entomopathogen; Nilaparvata lugens ABSTRAKWereng coklat telah dilaporkan resisten terhadap berbagai jenis insektisida. Salah satu pengendalian yang ramah lingkungan, yaitu dengan penggunaan jamur entomopatogen. Penelitian ini bertujuan untuk menguji keefektifan jamur entomopatogen M. huainamdangense isolat lokal terhadap wereng coklat. Pengujian patogenisitas isolat lokal M. huainamdangense menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan yaitu perlakuan K1 (Kerapatan 106), K2 (Kerapatan 107), K3 (Kerapatan 108), K4 (Kerapatan 109) dan K0 sebagai kontrol. Setiap perlakuan diulang 5 (lima) kali. Hasil penelitian menunjukkan bahwa perlakuan yang paling cepat mematikan serangga hingga 100% yaitu pada perlakuan K4 (Kerapatan 109). Hasil analisis probit menunjukkan nilai LT50 pada perlakuan kerapatan konidium 109 (K4) yaitu pada 1,6 hari, sedangkan nilai LC50 menunjukkan konsentrasi 108,9 konidium/ml pada hari kedua. Penggunaan konsentrasi kerapatan konidia yang lebih rendah mampu mematikan 50% serangga dalam waktu yang lebih lama. Kata kunci: Metarhizium huainamdangense; entomopatogen; Nilaparvata lugens