Antibiotic resistance of Staphylococcus-like organisms (STLO) isolated from a recreational sea beach located on the southern coast of the Baltic Sea was studied. The results of the present study showed that STLO inhabiting sand and seawater of the beach strongly differed in the resistance level to tested antibiotics. These microorganisms were most resistant to ampicillin, penicillin, oxytetracycline and susceptible to gentamicin, neomycin and streptomycin. Moreover, the level of antibiotic resistance among bacteria isolated from different parts of the beach also differed. Bacteria inhabiting the seawater, shoreline and the middle part of the beach were more antibioticresistant than bacteria isolated from the dune. The majority of bacteria inhabiting the seawater and sand were resistant to 3-8 antibiotics out of 12 tested in this study. Generally, there was no difference in antibiotic resistance between Staphylococcus-like