Energy cane varieties are high-fiber sugarcane clones which represent a promising feedstock in the production of alternative biofuels and biobased products. This study explored the crop establishment and whole farm production costs of growing energy cane as a biofuel feedstock in the southeastern USA. More specifically, total production costs on a feedstock dry matter biomass basis were estimated for five perennial energy cane varieties over alternative crop cycle lengths. Variable production costs for energy cane production were estimated to be in the $63 to $76 Mg −1 range of biomass dry matter for crop cycles through harvest of fourth through sixth stubble crops. Total production costs, including charges for fixed equipment costs, general farm overhead, and land rent, were estimated to range between $105 and $127 Mg −1 of feedstock biomass dry matter material.