Control of calcium-mediated storage defects, such as age gelation and sedimentation, were evaluated in enteral high-protein dairy beverages during storage. To investigate the effects of reduced-calcium ingredients on storage stability, 2 batches each of milk protein concentrates (MPC) with 3 levels of calcium content were acquired [control,, and 30% calcium-reduced (MPC-30)]. Control and calciumreduced MPC were used to formulate 8% (wt/wt) protein enteral dairy beverages. The formulation also consisted of other ingredients, such as gums, maltodextrin, potassium citrate, and sucrose. The pH-adjusted formulation was divided into 2 parts, one with 0.15% sodium hexametaphosphate (SHMP) and the other with 0% SHMP. The formulations were homogenized and retort sterilized at 121°C for 15 min. The retortsterilized beverages were stored at room temperature for up to 90 d and particle size and apparent viscosity were measured on d 0, 7, 30, 60, and 90. Beverages formulated using control MPC with 0 and 0.15% SHMP exhibited sedimentation, causing a decrease in apparent viscosity by approximately 10 cP and clear phase separation by d 90. The MPC-20 beverages with 0% SHMP exhibited stable particle size and apparent viscosities during storage. In the presence of 0.15% SHMP, particle size increased rapidly by 40 nm on d 90, implying the start of progressive gelation. On the other hand, highest apparent viscosities leading to gelation were observed in MPC-30 beverages at both concentrations of SHMP studied. These results suggested that beverages formulated with MPC-20 and 0% SHMP would have better storage stability by maintaining lower apparent viscosities. Further reduction of calcium using MPC-30 resulted in rapid gelation of beverages during storage.

An obligate heterofermentative, Lactobacillus wasatchensis has been recently isolated from an aged Cheddar cheese produced in Logan, Utah. The potential of this organism in causing gassing defects in aged cheese has raised concern among cheese manufacturers. The recent attention on this organism is attributed to its economic impact due to low-quality cheese. This comprehensive review provides the details about Lb.wasatchensis characteristics, geographical distribution and effect of various physical and chemical factors such as heat treatment, carbohydrate utilization, pH, salt tolerance and growth temperature. Lb. wasatchensis utilize ribose as a primary source for its growth, however, it can slowly utilize galactose resulting in gas generation. The details of testing methods along with suggestions for future research on improving these techniques using a phage as a selective medium are provided in this review. Recent research developments for controlling the growth of Lb. wasatchensis, as well as potential research opportunities are summarized in this review.

Thermoduric bacteria are known to affect the quality of Cheddar cheese, with manifested defects including slits, weak body, and blowing. Thermoduric bacteria are likely to increase in numbers during cheese-making, as in-process conditions are conducive to proliferation. The present study was conducted to track thermoduric bacterial progression during an 18-to 20-h Cheddar cheese production run and during ripening when the pasteurizer was washed at midway through the production day. This study also correlated a broad range of chemical changes to the growth of thermoduric bacteria during ripening. Three independent cheese trials were performed at 3.5-± 0.5-mo intervals. Samples were drawn in duplicates at 4 different times of the day: at the start of the run (vat 1), prior to a midday wash of the pasteurizer (vat 20), after the midday wash of the pasteurizer (vat 21), and at the end of the run (vat 42) for raw milk, pasteurized milk, and cheese. Cheeses were also tested during ripening for 6 mo. Results showed that raw milk total bacterial counts comprised 0.24% thermoduric mesophiles (TM) and 0.12% thermoduric thermophiles (TT). The thermoduric thermophilic bacterial counts increased by log 10 1.23 during the pasteurizer run of 9 to 10 h, indicating a buildup of thermoduric thermophilic bacteria during the pasteurization process itself. Midday washing reduced thermophilic counts by log 10 1.36, as evident by pre-and post-midday wash counts. However, a thermophilic buildup during post-midday wash was again noticed near the end of the 20-h run. We found that TT bacteria decreased in the first 60 d of ripening, whereas TM bacteria increased during the same period. However, TT bacteria increased later during 60 to 180 d of ripening. Bacillus licheniformis was the most frequently isolated bacteria in this study and was recovered at all production stages sampled during the cheese-making and ripening. We observed a significant increase in the level of orotic and uric acids in the vat made at the end of the day. No significant difference in the overall chemical composition, proteolysis, sugar, or other organic acids was observed in cheese made at the start versus the end of the production run.