Currently the use of nanotechnology has been seeking to develop additives that have antimicrobial and low toxicity, enabling applications in various industrial sectors, in particular the paint industry. Inks with antimicrobial properties enable greater security to the user, eliminating pathogens that can cause disease when in contact with humans and animals. Thus, the zinc oxide nanoparticles have attracted a great scientific and technological interest as inorganic antimicrobial agent due to its oligodynamic effect. This work aims to study the effect oligodynamic zinc oxide nanoparticles in antimicrobial properties of acrylic paints. The acrylic paints were developed with various concentrations of zinc oxide nanoparticles, and were subsequently conducted microbiological characterization of death curve and agar diffusion. To evaluation the physico-chemical properties were used the techniques differential scanning calorimetry (DSC); Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The results demonstrate that there was a reduction in the high antimicrobial activity of paints containing zinc oxide nanoparticles as compared to standard paint. Even with the addition of zinc oxide nanoparticles did not change the microstructure and chemical properties of acrylic coatings.