: To study the antibiogram of Klebsiella, Pseudomonas and Staphylococcus and its change in sensitivities with time. : 4909 swabs were taken from790 of patients admitted to the Burns unit of Masina Hospital, Mumbai, over a period of 12 years (2008–2019). The swabs were cultured and percentage antibiotic sensitivity of 6835 predominate isolates to different class of antibiotic was determined and reviewed. : Klebsiella was the predominant organism in our set-up, followed by Pseudomonas and Staphylococcus aureus. The antibiotic sensitivities of the most predominant organisms are discussed in detail in this article. : Gram negative nosocomial infection predominate a burn injury. Knowing the predominant target pathogens and their sensitivity pattern towards different antibiotics will avoid misuse of antibiotic, contribute to prescribing the correct antibiotics and timely clinical treatment. A routine microbiological surveillance prior to administrating an antibiotic, a well established infection control department and regular reporting of changing antibiotic trends will help us overcome our battle against emerging multi drug resistant organism, thereby having more successful treatment outcome in burn patients.