A series of novel 4-hydroxycoumarin derivatives 4a-n were synthesized by treating 2-(2-oxo-2H-chromen-4-yloxy)acetic acid 3 with various amines. Acid 3 was obtained from the hydrolysis of corresponding ester 2 which was prepared from 4-hydroxycoumarin 1 and ethyl bromoacetate. All synthesized compounds 4a-n were characterized using spectral techniques. The synthesized compounds 4a-n were evaluated for their antimicrobial and total antioxidant activity using in vitro well diffusion method and phosphomolybdenum method, respectively. Amongst all the compounds, 4c has shown potential antimicrobial and antioxidant activity against the standard. All compounds have exhibited good binding energies from -4.46 to -5.70 kcal/mol against 24-kDa DNA gyrase B fragment from E. coli (PDB ID-1KZN) in molecular docking study and amongst them 4c has shown maximum binding energy.

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One pot synthesis with good yields. Good antimicrobial activity against 4EMV receptor. Prominent anticancer activity against A549 and SKOV-3 cell lines. Significantin vitrocytotoxicity at 7.81 μg mL−1. Docking mode of1hwith 2XP2 receptor.

In vitro and in vivo anticancer activity of compound 3a was proved as a novel blocker of JAK2/STAT3 signaling pathway and exerts both anti-proliferative and apoptotic activities in HepG-2 cells with xenograft mice model.