The genetic variability studies indicated that the prevalence of sufficient genetic variation among the genotypes for all the characters. The high Genotypic co-efficient of variation and Phenotypic co-efficient of variation were observed for the number of primary branches and number of fruits per plant. High heritability coupled with high Genetic Advance as per cent of Mean was observed for plant height, number of primary branches, plant spread from North to South, plant spread from East to West, fruit length, fruit diameter, average fruit weight, number of fruits per plant, fruit yield per plant and Total Soluble Solids indicating that these characters are governed by additive gene action. Hence, direct selection may be followed for the improvement of brinjal by assessing these characters.
Identification of root(wilt) disease-free palm is a basic requirement for evolving disease resistant/tolerant planting materials for the management of this phytoplasma induced malady. For this purpose, various types of ELISA were performed with different enzymes and their respective substrates to standardize the most suitable and sensitive one. Indirect ELISA, Protein A indirect ELISA and F(ab')2 indirect ELISA using Alkaline Phosphatase conjugate and DAC indirect ELISA using Horse raddish peroxidase conjugate were carried out. Of the various types of ELISA tried, DAC indirect ELISA has been found to be the best for the rapid detection of coconut root(wilt) disease. In this assay, crude leaf extracts and unfractionated polyclonal antiserum were employed as test antigen and specific antibody respectively. Higher specificity was observed with the addition of gelatin and ovalbumin in the extraction medium and overnight incubation of ELISA plate at 5°C after substrate addition. Antigen titre was found to be very high in spear leaves followed by the next outer leaf. Similarly, maximum antigen titre was observed during the early stage of the diseased palm. The test could be completed within 44h and in a single ELISA plate, 20 samples with three replications could be screened using microlitre quantities of the specific antibody.
The experiment was undertaken to study the correlation and path analysis in 300 F2 segregating population of the bi-parental cross Surya × Harita for 12 different quantitative characters. The correlation study indicated that the total yield per plant had a positive and significant association with plant height, number of primary branches, number of flowers per cluster, number of fruits per cluster, fruit diameter, fruit length, average fruit weight and number of fruits per plant. The genetic improvement of fruit yield can be obtained by selection of these yield components. Path analysis revealed that the characters number of fruits per plant, average fruit weight, plant height, fruit length, fruit set percentage, fruit diameter, number of flowers per cluster, days to first picking had high positive direct effect on fruit yield per plant. Thus, the fruit yield per plant can be improved by making direct selection of these characters.
The experiment was conducted in order to estimate the character association for eleven traits in thirty diverse garden pea genotypes during Rabi season of 2018-19 at the Research Farm, Department of Vegetable Science, College of Horticulture, Mudigere. Quantitative analysis were carried out for all the characters which are directly or indirectly associated with the yield and yield contributing traits. The correlation studies revealed that pod yield per plant exhibited highly positive and significant character association with number of pods per plant and weight of ten pods. Therefore, these traits may be considered as the most reliable selection indices for effective improvement in yield.
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