No abstract

assay and circulating experiments were performed with an experimental setup with a peristaltic pump. Results and discussions In our studies, HOTAIR expression is found to be reversely correlated with c-Met expression and activation in HCC cell lines, for the first time.c-Met activation leads to down-regulation of lncRNA HOTAIR expression and also, inhibition of c-Met activation recovers HOTAIR expression. c-Met pathway is defined to be activated in liganddependent and independent manner. Reciprocal interaction of c-Met and HOTAIR was conserved in ligand-independent cMet activation,too.Bioinformatics analysis of HOTAIR regulated genes highlighted some genes that are known to contribute to c-Met pathway via enabling its activation.The possible molecules that take part in reciprocal interaction of c-Met and HOTAIR are analysed by qPCR and western blot. Our findings are the first to show Caveolin-1, a membrane protein, is an important target of HOTAIR to regulate c-Met pathway and also Vimentin is regulated by HOTAIR expression to mediate epithelial/mesenchymal phenotype in HCC.To further understand the role of HOTAIR and c-Met interaction in metastasis; expression of HOTAIR, c-Met and related molecules were analysed in circulating HCC cells under shear stress. Conclusion Reported data revealed that c-Met pathway related mesenchymal phenotype and adhesion-independent cell growth requires HOTAIR downregulation which leads to increase of Caveolin-1 expression.Here we report that HOTAIR downregulation is a requirement to survive in circulation, adhesion independent survival and growth and it is regulated by c-Met receptor tyrosine kinase activity.Our results contribute to the literature by mapping the interaction of HOTAIR and c-Met. Introduction Nuclear factor-kB (NF-kB) plays a pivotal role in inflammation and innate immunity and aberrant regulation of NF-kB can influence cancer development and progression (Ref 1). As a result, a great deal of research is now focused on targeting this signalling pathway therapeutically. In order to accurately and reproducibly examine the NF-kB signalling cascade there is a need for highly specific antibodies. We, at Abcam, have developed knockout (KO)-validated antibodies targeted to distinct elements of the NF-kB signalling pathway to maximise experimental accuracy and reproducibility. Material and methods Our KO validation program uses the extensive library of KO cell lines available from Horizon Discovery. Target genes are mutated via CRISPR-Cas9 within a haploid cell line, resulting in a frameshift and complete loss of gene expression. Target specificity is then confirmed via western blot, flow cytometry or immunocytochemistry. These KO cell lines function as true negative controls that can be used at a large scale to confirm antibody specificity to target proteins. Results and discussions We present antibody validation data for seven targets in the NF-kB signalling pathway. Of the 30Abcam products tested, we demonstrate that 22 antibodies are specific. The remaining 8 have since...

The rate of iron corrosion in a low-temperature plasma of moist air formed under the action of a fast electron beam, depending on the relative air humidity, was measured. It is shown that a beam of fast electrons (radioactive β-radiation) significantly intensifies the corrosion of iron in a plasma-forming gas medium in which oxygen and water vapour are simultaneously present. It has been established that the rate of corrosion under conditions of radioactive irradiation increases sharply when the relative air humidity exceeds 10%. Numerical simulation of the ionic composition of the plasma is carried out taking into account 12 types of positive and 12 types of negative hydrated ions at different intensities of the external ionization source and at different values of relative humidity from 10–6 to 100%. A hypothesis has been put forward about the determining role of cluster hydrated ions, which are formed in the plasma of moist air at atmospheric pressure, in heterogeneous processes of iron oxidation.

No abstract