the toxic and genotoxic effects of silver nanoparticles were studied on injected mice (BALB/c line) in vivo. A water solution of silver nanoparticles (SnP) with particle sizes of 9±6 nm was obtained by means of the original method of biochemical synthesis. the effect of the SnP solution was compared to those of AOt (anionic surfactant used as SnP stabilizer) and silver nitrate (i.e. Ag+ ions) introduced as water solutions. In studies of the toxic effects, the death of mice was registered 12-24 hours after injection only at two maximum dozes of SnP (equivalent to 0.54 and 0.36 gAg/l). It is shown that the toxic effect decreases in the sequence SnP>AOt>>AgnO3. the Le50/30 values for SnP and AOt are equal to 0.30±0.07 gAg/l and 13.3±2.1 gAg/l, respectively. Genotoxic effects were assessed by the abnormal sperm heads test and neutral comet assay. the frequencies of abnormal sperm heads (ASHs) did not differ after treatment by SnP and AOt, but both were significantly higher than those found with AgnO3 and in control mice. comet assay showed an increase of the DnA percentage in the comet tail in spleen cells after the injection of SnP and AOt in concentrations of ½ Le50/30. tail DnA % was 32.8±1.3 and 26.3±1.7%, respectively, vs 16.2±0.7% for the untreated control. to sum up, these tests showed that the genotoxic effects of the SnP solution are associated with the presence of AOt rather than SnP.

The toxic and genotoxic effects of silver nanoparticles were studied on injected mice (BALB/c line) in vivo. A water solution of silver nanoparticles (SNP) with particle sizes of 9±6 nm was obtained by means of the original method of biochemical synthesis. The effect of the SNP solution was compared to those of AOT (anionic surfactant used as SNP stabilizer) and silver nitrate (i.e. Ag+ ions) introduced as water solutions. In studies of the toxic effects, the death of mice was registered 12-24 hours after injection only at two maximum dozes of SNP (equivalent to 0.54 and 0.36 gAg/l). It is shown that the toxic effect decreases in the sequence SNP>AOT>>AgNO3. The LE50/30 values for SNP and AOT are equal to 0.30±0.07 gAg/l and 13.3±2.1 gAg/l, respectively. Genotoxic effects were assessed by the abnormal sperm heads test and neutral Comet assay. The frequencies of abnormal sperm heads (ASHs) did not differ after treatment by SNP and AOT, but both were significantly higher than those found with AgNO3 and in control mice. Comet assay showed an increase of the DNA percentage in the comet tail in spleen cells after the injection of SNP and AOT in concentrations of ≤ LE50/30. Tail DNA % was 32.8±1.3 and 26.3±1.7%, respectively, vs 16.2±0.7% for the untreated control. To sum up, these tests showed that the genotoxic effects of the SNP solution are associated with the presence of AOT rather than SNP.

The combined action of ceria nanoparticles and doxorubicin on the early stages of ontogenesis of Danio rerio and Puntius tetrazona was studied. Results obtained indicate that there is a synergetic effect of CeO 2 nanoparticles and doxorubicin which is demonstrated by a high incidence of embryonic malformations in fish. This synergetic effect is more pronounced in tiger barbs than in zebrafish, and depends strongly on the synthetic route of ceria nanoparticles' preparation, the most notable effects being registered for citrate-stabilized nanoparticles.

No abstract

No abstract