Microcystis aeruginosa PCC 7806 produces potent inhibitors of eukaryotic protein phosphatases called microcystins, whose function to the organism is presently unknown. Mutants with impaired microcystin biosynthesis should provide a useful tool for investigations of microcystin function. This study has focussed on the comparison of growth and pigment content of strain PCC 7806 and its mcyB− mutant deficient in microcystin biosynthesis, under semicontinuous culture conditions. Both wild‐type and mutant are characterised by a very low light demand and low‐maximum specific growth rates in comparison to other Microcystis strains studied. While growth of wild‐type and mutant were similar under different light conditions, the mutant cells showed significantly higher specific absorbances in the range of photosynthetic active radiance 420–700 nm, under light‐limiting conditions. The mutant cells possess lower contents of chlorophyll a, β‐carotene, zeaxanthin and echinenone under light limitation and of myxoxanthophyll under saturated light conditions. Though microcystins are clearly not essential for growth, the observed effects of the mutation are a first indication of their involvement in intracellular processes.

Various sites of Phragmites australis in Germany (Uckermark and Mark Brandenburg) and sites in Hungary and Denmark were investigated by PCR fingerprinting in order to determine their genetic diversity. Genetic similarities were calculated and. based on this, a cluster analysis was performed. The genetic similarity of reed clones around one lake ranged from between 70 to loo%, that of clones from different lakes was clearly lower. The further apart the clones were situated from each other, the less similar they were. At a wetland site near the shore a sudden colonisation by several new Phragmites clones was observed. This site was located in front of a well established genetically almost homogenous stand within the eulittoral. All the results are discussed in connection with a proposed model of establishment and development of water reed stands: colonization, selection of best adapted clones by competition and the stability of stands.

Microcystis aeruginosa PCC 7806 produces potent inhibitors of eukaryotic protein phosphatases called microcystins, whose function to the organism is presently unknown. Mutants with impaired microcystin biosynthesis should provide a useful tool for investigations of microcystin function. This study has focussed on the comparison of growth and pigment content of strain PCC 7806 and its mcyB 3 mutant deficient in microcystin biosynthesis, under semicontinuous culture conditions. Both wild-type and mutant are characterised by a very low light demand and low-maximum specific growth rates in comparison to other Microcystis strains studied. While growth of wild-type and mutant were similar under different light conditions, the mutant cells showed significantly higher specific absorbances in the range of photosynthetic active radiance 420^700 nm, under light-limiting conditions. The mutant cells possess lower contents of chlorophyll a, L-carotene, zeaxanthin and echinenone under light limitation and of myxoxanthophyll under saturated light conditions. Though microcystins are clearly not essential for growth, the observed effects of the mutation are a first indication of their involvement in intracellular processes. ß