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Various factors affecting the estimation of the tryptophan content of wool, by methods based on acid hydrolysis, are discussed. It has been shown that when wool is hydrolyzed in a strong acid medium in presence of p-dimethylaminobenzaldehyde, the tryptophan-aldehyde complex formed decomposes with time and, thus, a correct estimation of tryptophan content is difhcult. When wool is hydrolyzed with barium hydroxide, tryptophan is liberated without decomposition and can be estimated in the hydrolyzate when barium ions and H2S (which is formed as a result of degradation of cystine and its derivatives) are both removed. A method of estimating tryptophan content of wool by baryta hydrolysis is described and has been found to be accurate and reliable. Tryptophan contents of various wools, estimated by the baryta hydrolysis method are reported. Tryptophan content of Australian Merino 70° wool was found to be 0.82 ± 0.01%.

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