Fatty acid methyl ester (FAME) extracts of four halophytic plants, viz. Arthrocnemum indicum, Salicornia brachiata, Suaeda maritima and Suaeda monoica belonging to the family Chenopodiaceae, were prepared and their composition was analyzed by GC-MS. The FAME extracts were also screened for antibacterial and antifungal activities. The GC-MS analysis revealed the presence of more saturated fatty acids than unsaturated fatty acids. Among the fatty acids analyzed, the relative percentage of lauric acid was high in S. brachiata (61.85%). The FAME extract of S. brachiata showed the highest antibacterial and antifungal activities among the extracts tested. The other three extracts showed potent antibacterial and moderate anticandidal activities.
The essential oil from the leaves of Blumea mollis was extracted and the chemical constituents and the larvicidal against Culex quinquefasciatus effects studied. The analyses of gas chromatography (GC) and GC-mass spectroscopy (GC-MS) revealed that the leaf essential oil of B. mollis contained 39 compounds, and the major chemical compounds identified were linalool (19.43%), gamma-elemene (12.19%), copaene (10.93%), estragole (10.81%), Allo-ocimene (10.03%), gamma-terpinene (8.28%) and Allo-aromadendrene (7.44%). The essential oil had significant toxic effect against early fourth-instar larvae of C. quinquefasciatus with LC50=71.71 and LC90=143.41 ppm. The results could be useful in search of newer, safer and more effective natural larvicidal agent against C. quinquefasciatus.
The early fourth instar larvae of Culex quinquefasciatus, reared in the laboratory were used for larvicidal assay with leaf extracts of Vitex negundo, Vitex trifolia, Vitex peduncularis and Vitex altissima. The methanol extracts of the four species possessed varying levels of larvicidal nature. The highest larvicidal activity was found with the extract of V. trifolia (LC(50) = 41.41 ppm) followed by V. peduncularis (LC(50) = 76.28 ppm), V. altissima (LC(50) = 128.04 ppm) and V. negundo (LC(50) = 212.57 ppm).
The larvicidal activity of fatty acid methyl ester (FAME) extracts of Vitex altissima, Vitex negundo and Vitex trifolia was studied against early fourth-instar larvae of Culex quinquefasciatus. The fatty composition was analysed by gas chromatography. The highest percentage of lauric acid, palmitic acid, stearic acid, oleic acid and linolenic acid were recorded in V. negundo. The highest percentage of linolenic acid was recorded in V. trifolia. The FAME extract of V. trifolia showed the highest larvicidal activity with an LC50 value of 9.25 ppm followed by V. altissima (14.82 ppm) and V. negundo (18.64 ppm).
The antibacterial activity of aqueous and methanol extracts of leaves/shoots of five salt marsh halophytes and six mangroves was studied against methicillin resistant, clinical isolates of Staphylococcus aureus. There was a clear comparability between the salt marsh halophytes and mangroves in their antibacterial action. The mangrove plants possessed higher antibacterial potency than the salt marsh halophytes. The highest activity was recorded with the methanol extract of Excoecaria agallocha followed by the methanol extracts of Aegiceras corniculatum, Lumnitzera racemosa and Ceriops decandra. The minimum inhibitory concentration (MIC) values ranged from 0.125 to 4 mg/mL and 1 to 16 mg/mL for methanol and aqueous extracts, respectively. Further separation of active principle from the potent mangrove plant will be useful for the control of drug resistant strains of S. aureus.
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