SummaryChromatin modifying activities inherent to polycomb repressive complexes PRC1 and PRC2 play an essential role in gene regulation, cellular differentiation, and development. However, the mechanisms by which these complexes recognize their target sites and function together to form repressive chromatin domains remain poorly understood. Recruitment of PRC1 to target sites has been proposed to occur through a hierarchical process, dependent on prior nucleation of PRC2 and placement of H3K27me3. Here, using a de novo targeting assay in mouse embryonic stem cells we unexpectedly discover that PRC1-dependent H2AK119ub1 leads to recruitment of PRC2 and H3K27me3 to effectively initiate a polycomb domain. This activity is restricted to variant PRC1 complexes, and genetic ablation experiments reveal that targeting of the variant PCGF1/PRC1 complex by KDM2B to CpG islands is required for normal polycomb domain formation and mouse development. These observations provide a surprising PRC1-dependent logic for PRC2 occupancy at target sites in vivo.

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The first measurement of transverse-spin-dependent azimuthal asymmetries in the pion-induced Drell-Yan (DY) process is reported. We use the CERN SPS 190  GeV/c π^{-} beam and a transversely polarized ammonia target. Three azimuthal asymmetries giving access to different transverse-momentum-dependent (TMD) parton distribution functions (PDFs) are extracted using dimuon events with invariant mass between 4.3  GeV/c^{2} and 8.5  GeV/c^{2}. Within the experimental uncertainties, the observed sign of the Sivers asymmetry is found to be consistent with the fundamental prediction of quantum chromodynamics (QCD) that the Sivers TMD PDFs extracted from DY have a sign opposite to the one extracted from semi-inclusive deep-inelastic scattering (SIDIS) data. We present two other asymmetries originating from the pion Boer-Mulders TMD PDFs convoluted with either the nucleon transversity or pretzelosity TMD PDFs. A recent COMPASS SIDIS measurement was obtained at a hard scale comparable to that of these DY results. This opens the way for possible tests of fundamental QCD universality predictions.

The COMPASS Collaboration at CERN has measured the transverse spin azimuthal asymmetry of\ud charged hadrons produced in semi-inclusive deep inelastic scattering using a 160 GeV μ+ beam and a\ud transversely polarised NH3 target. The Sivers asymmetry of the proton has been extracted in the Bjorken\ud x range 0.003 < x < 0.7. The new measurements have small statistical and systematic uncertainties of\ud a few percent and confirm with considerably better accuracy the previous COMPASS measurement. The\ud Sivers asymmetry is found to be compatible with zero for negative hadrons and positive for positive\ud hadrons, a clear indication of a spin–orbit coupling of quarks in a transversely polarised proton. As\ud compared to measurements at lower energy, a smaller Sivers asymmetry for positive hadrons is found\ud in the region x > 0.03. The asymmetry is different from zero and positive also in the low x region,\ud where sea-quarks dominate. The kinematic dependence of the asymmetry has also been investigated and\ud results are given for various intervals of hadron and virtual photon fractional energy. In contrast to the\ud case of the Collins asymmetry, the results on the Sivers asymmetry suggest a strong dependence on the\ud four-momentum transfer to the nucleon, in agreement with the most recent calculations

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