Adequate pulmonary development at birth is the major determinant of postnatal outcome in the perinatal period. Lung hypoplasia is a poorly defined condition. The aim of this study was to investigate expression of Ki-67 in human fetuses with pulmonary hypoplasia compared to fetuses without pulmonary pathology and malformations of other organs used as controls. The analysis comprised 149 formalin-fixed and paraffin-embedded tissue sections from the files of the Clinical Pathology Department of the Research Institute of Polish Mother's Memorial Hospital in Lodz. Tissue sections obtained from lungs during autopsies were divided into two groups. In our studies immunohistochemistry was performed using antibody against Ki-67 as a cell proliferation marker for evaluation of growth fraction in the fetal and neonatal human lungs. The results presented in our study showed higher expression of growth fraction in the control group as compared to study subjects in all stages of lung development. Values of Ki-67 positive cells in the saccular stage of lung development were lower than in the canalicular and alveolar phase in both study and control groups. In conclusion, our results indicate their usefulness to understand better etiology of pulmonary hypoplasia and may be helpful in identifying the most appropriate moment for prenatal interventions.

22nd World Congress on Ultrasound in Obstetrics and Gynecology Poster abstracts any of the twins which was confirmed on USG. Both babies were euthyroid at birth had normal development on follow up so far. Conclusion: Diagnosis of fetal hypothyroidism needs to be established at an early stage and appropriate hormone replacement treatment should be started. Fetal thyroid color Doppler is a new modality that helps in determining fetal thyroid function and can help to avoid invasive procedures like cordocentesis in appropriate cases. We emphasize on importance of noninvasive USG and maternal biochemical monitoring and correction as required. Hence, a strategic approach to fetal goiter is required to achieve optimal benefits at minimal fetal and maternal risk.Supporting information can be found in the online version of this abstract.Objectives: Congenital diaphragmatic hernia (CHD) is a defect, in which the abnormal development of the diaphragm leads to displacement of abdominal organs into the chest cavity and consequently to pulmonary hypoplasia. The development of prenatal diagnostics and fetal surgery in selected fetuses has improved the prognosis. Lung-to-head ratio (LHR), determined as the proportion between the lung situated contralateral to the loss in the diaphragm, to the fetal head circumference is of great importance as a prognostic factor. The goal of this study was to compare LHR in fetuses with CDH with pathomorphological parameters of lungs in newborns. Methods: In the study we included 52 patients hospitalized in the Department of Fetal-Maternal Medicine and Gynecology RIPMMH in Lodz between 2007 and 2012. CHD was diagnosed prenatally in ultrasound and MRI examinations. The fragments of lungs obtained during autopsies from the neonates who died were also studied as well as macroscopic and microscopic parameters of the lungs. Diagnostic criterion of pulmonary hypoplasia was the lung weight/body weight ratio (LW/BW). Results: Totally, from 52 infants with CHD: 34 died (65.4%) and 18 survived (34.6%). LHR ratio was calculated in 25 (48%) fetuses. LW/BW ratio was identified in 10 (62.5%) autopsies. The presence of the liver in the chest was found in 18 (34.6%) fetuses, including 13 (38.2%) who died and 5 (27.8%) who survived. Other defects were detected in 15 (28.8%) fetuses: 12 (35.3%) who died in the neonatal period and 3 (16.7%) who survived. Birth weight ≤ 2500 g was found in 13/52 (25%) infants who died and in 2/52 (3.8%) who survived. Less than 6 points in Apgar score received 22 (42.3%) newborns, of which 4 (7.7%) survived and 18 (34.6%) died. Conclusions:The initial results indicate a correlation between the assessed prenatally biometric parameters of fetal lungs, and measurements obtained during pathomorphological examination. P09.09 Successful treatment of fetal bilateral primary chylothorax

requiring parenteral nutrition and died neonatally. Megacystitismicrocolon-intestinal hypoperistalsis was diagnosed at post-mortem examination. Prune belly and megacystitis-microcolon-intestinal hypoperistalsis could co-exist and the prognosis may be more guarded. P22.12 Fetal edema complicated by mirror syndrome in the course of human parvorius B19 infection: a case report