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Abstract-Potassium dihydrogen phosphate is a material rich in NLO property with wide range of industrial applications. Certain Amino acids are also rich in NLO properties so they are used as dopants.In the present work LSerine a non essential amino acid is added in the ratio 1:1 molar percentage to KDP. Single crystals were grown by slow evaporation method. The grown crystals were characterized by powder X-ray diffraction, Single crystal X-Ray diffraction, UVvis-spectroscopy, Fourier transform infrared spectroscopy (FTIR), SHG efficiency and dielectric studies, The single crystal X-ray diffraction studies reveal the structure and space group of the crystal. The powder X-ray diffraction study shows that the crystalline perfection of grown crystal is good. Fourier transform infrared studies confirm the functional groups of the crystals. It is seen from UV-vis studies that the optical transparency is found to increase much by adding chlorides. SHG efficiency of KDP is found to be slightly increasing with doping of L-Serine.The electrical properties of the crystal have been studied using dielectric constant studies. All the results are compared with the results of pure KDP.