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Plants and microbes compete for survival and their organized interactions play a vital role in adapting to stress environments. Microbes have a positive impact on transplanting performance of in vitro propagated plantlets. The present study aims at survival development of micropropagated Pogostemon mollis through microbial inoculation. The leaf and stem explants on MS medium with different concentrations and combinations of BAP, Kn, NAA, IAA and IBA used for the establishment of plantlet. The survival rate of plantlets was successfully increased from 71% to about 70.21% by the inoculation of Rhizophagus fasciculatus and Bacillus megaterium. This inoculation also clearly increases the growth, biomass and chlorophyll of in vitro derived platelets. The present protocol emphasizes the need of bio-hardening on micropropagated plants, particularly the mycorrhization along with phosphobacteria.

Objective: A number of Indian medicinal plants have been used for thousands of years in a traditional system of medicine. Hemidesmus indicus is an important member of the Asclepiadaceae family. It is an endemic to the southern Western Ghats, India. The aim of the study was to investigate the free radical scavenging activity of H. indicus. Methods: The aqueous and methanol leaf extracts of H. indicus were assayed for radical scavenging activity, using the stable free radical 2,2-diphenyl- 1-picryl-hydrazyl-hydrate and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid). Results: The results revealed that the IC50 values of aqueous extract of H. indicus were found to be higher than that of the other solvent extracts. The free radical scavenging activity of the plant extracts may be due to the presence of phytoconstituents. Conclusion: In all the methods, the aqueous extract has exhibited the good scavenging activity and this arises that the plant has a potential antioxidant agent.

The plants provide a source of inspiration for novel drug compounds, as plant derived medicines made large contributions to human health and well being. The Present investigation is about the antibacterial activity of Breynia patens (Roxb.) Benth. & Hook.f. against five bacterial strains using the disc diffusion agarmethod. The whole plant extracted with ethanol, chloroform, petroleum ether and Aqueous. The sensitivity order of the Methanol crude extracts was illustrated by the corresponding inhibition zone diameter to be Salmonella paratyphii(20±009mm zone of incubation) and followed by other bacterial strains. The resultsindicated that the plant extract exhibited antibacterial properties, thus justifying scientifically their traditional uses as medicinal plants

The present investigation has brought out the AM fungal association in some plant species of Kodikuthimala, Malappuram district Kerala. Totally, 25 plant species belongs to 15 families were analyzed for arbuscular mycorrhizal association. The root samples of all collected plant species showed mycorrhizal infection. The percentage of colonization was varied with plant species and it ranges from 12 (Commelina benghalensis) to 79% (Sida rhombifolia). Maximum spore population was observed in Gloriosa superba (574/100g of soil) and minimum in Euphorbia hirta (143/10g of soil). Totally 26 AM fungal species belongs to 13 genera were found. Among this Glomus was most dominated. In most of the plants, spores of Rhizophagus fasciculatus are seen. Present study confirms the Arbuscular Mycorrhizal association in the collected plant species.