Abstract:Background: Majority of secondary school students are adolescents; an age group with a tendency to engage in healthcompromising risky behaviours in order to deal with anxiety and gain admission to peer groups. This exposes them to risk of Hepatitis B virus infection which is a major public health concern globally and in Nigeria.Objectives: This study focuses on some common risky behavioural practices that characterize the lifestyle of contemporary adolescent subculture; with a view to determining their effect on HBsAg seroprevalence. Method: Using multi-stage sampling method, 266 students were selected from 5 secondary schools in Abakaliki. Each subject first filled a questionnaire regarding biodata and history of exposure to risk factors. Subsequently, they were screened for HBsAg using enzyme immunoassay-based chromatographic test kit. Results:Nine students out of 266 tested positive giving a prevalence value of 3.38%. There was no significant association between gender (p = 0.31) or socioeconomic status (p = 0.81) and the seroprevalence of HBsAg among the subjects. Similarly, none of the risk factors studied, including previous history of jaundice (p = 0.26), blood transfusion (p = 0.24), past history of surgery (p = 0.47), scarification marks (p = 0.17), sharing of sharp objects (p = 0.74), drug injections (p = 0.32), unprotected sex (p = 0.64) and family history of hepatitis (p = 0.79), was significantly associated with HBV infection. Conclusion: None of the risk factors assessed played significant role in the transmission of HBV among secondary school students in Abakaliki.