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The HPLC separation of dansylated amides including mono-, di-, and polyamines from a Peucedanum palustre (L.) Moench plant and an embryogenic cell culture line was studied. The flowers, stems, leaves, and roots of the plant were analyzed separately. The mobile phase was optimized with the "PRISMA" model, which is a multi-solvent optimization system. Twenty-five standard amines were tested, twelve of which were present in the plant samples. The amine concentrations varied considerably in the different organs of the plant. There were also differences in amine concentrations between the wild plant and the embryogenic cell line and plantlets. The "PRISMA" optimization system proved to be a rapid and efficient method in the HPLC analysis of biogenic amines present in plant samples.

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Arnines are organic bases which are often derived from amino acids by decarboxylation. A wide variety of amines occurs in the plant kingdom where they may regulate the growth or be the part of the mechanism to attract the pollenating insects. The distinction between alkaloids and amines is sometimes not well defined and some true amines are often referred to as alkaloids. However, alkaloids almost invariably have the N atoms in heterocyclic groups, and they usually have a higher molecular mass than amines. Amines are precursors for several alkaloids. Plants in the Apiaceae and Solanaceae families, e.g., Peucedanum palustre (L.) Moench, Atropa belladonna (L.), and Nicotiana tabacum (L.) contain several amines including aliphatic, di-and polyamines. These amines play an important role in the biosynthesis of secondary metabolites and, therefore, it is important to study their chromatographic behaviour in order to establish their analytical characteristics.The aim of the present study was to investigate the retention and molecular structure dependent behaviour of dansylated amines in liquid chromatography.Thirteen closely related dansyl amides were studied in isocratic reversed-phase HPLC using the "PRISMA" optimization system (1). Tetrahydrofuran, acetonitrile, and methanol in water were selected to give the best separation of the compounds. The retention measurements were performed in sixty one selectivity points (P5) using one (n = 3), two (n = 27), or three (n = 31) of the selected solvents, water serving as the solvent strength (ST) regulator. 5T of the solvent combinations was adjusted to 1.7 to give the last eluting compound a reasonable band width and the capacity factor (k') of 30. The quadratic, cubic, and fourth order regression functions of the measured retention data were studied. The molecular structures of the compounds were described by the molecular connectivity indices (2). These indices reflect the shape and atomic interactions of a molecule. 28 different types of indices including path, cluster, and path/cluster types were calculated for the compounds.The study showed that high correlation coefficients (r = 1.00-0.96) for the dependence between the P5 and k' values of the dansyl amines expressed as functions were obtained. The cubic and fourth order regression functions described the correlation best for the different solvent combinations. The molecular structure and the retention behaviour of the compounds had a clear dependence in isocratic reversed-phase HPLC.The compounds were divided into two groups on the basis of different behaviour in HPLC. The group I contained dansylated monoamines with increasing carbon chains. The group II contained mainly diamines with bulky substituents. The lower order indices described best the structure related be-haviour. The zero order valence level indices (0f) were selected for all amines. When the higher order indices and indices which describe the branching of the molecule, e.g. path/ cluster types, were studied, the correlation with the amines decreased.The...

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