Breast milk is essential food for a baby's health. Many factors influence the success of breastfeeding, one of which is maternal anxiety. The infant mortality rate in Tegal is the highest in Central Java. The problem of breastfeeding supports the high rate of infant mortality. This study aims to determine the relationship between postpartum primparous mothers' anxiety level and breast milk ejection in the first 24 hours at Harapan Anda Islamic General Hospital in Tegal. This crosssectional analytic study was followed by 65 respondents who met the inclusion and exclusion criteria. The sampling technique used was simple random sampling. Anxiety data was measured using the Hamilton Anxiety Rating Scale (HARS). The bivariate analysis used the chisquare test to determine the relationship between anxiety level and breast milk ejection in the first 24 hours. There were 43 respondents who were unable to eject breast milk in the first 24 hours (66.2%), while respondents who experienced anxiety and were unable to eject breast milk in the first 24 hours were 41 people (95.3%). The results of statistical tests using the chi-square method showed significant difference in breastfeeding ejection success in the two groups (p <0.05). It indicated that there was a relationship between anxiety in postpartum primiparous mothers and breast milk ejection in the first 24 hours.