Abstract. Hypersensitivity of teeth pose an ineresting question in the practice of the dentist because of its high prevalence and polyetiological nature. The aim of the work was to establish the incidence of tooth hyperesthesia among elderly residents of Vasileostrovsky and Petrogradsky districts of St. Petersburg. The study involved 117 participants of both sexes, divided into 2 age groups (55-64;65-75). 70 patients with hypersensitivity of teeth tissues were detected. In addition to establishing the fact of hyperesthesia, patients were interviewed, teeth were examined for the presence of carious and non-carious lesions, the states of oral hygiene and periodontal tissues were evaluated using dental indices. To determine the general condition of the patient's oral health we used: OHI (simplified Green-Vermillion index, (Green, Vermillion, 1964)) - to determine the level of oral hygiene, PI index (P.A. Leus, 1988) - to determine the presence of symptoms of periodontal pathology, DMF (Klein, Palmer, 1937) - to determine the degree of dental caries. Assessment of dental hyperesthesia was carried out using indices of prevalence (IPDH) and intensity (IIDH) of dental hyperesthesia. According to the results of the examination the following conclusions were made: the most susceptible to hyperesthesia were women from 55 to 64 years old (44,28% of all those examined). An unsatisfactory level of hygiene, an average degree of periodontal disease and a high degree of caries intensity were detected, a limited form of hyperesthesia was more frequent (56.81% of all cases) with a reaction to all types of stimuli(63.02% of all cases), a moderate level of tooth sensitivity efficiency was noted(56.5%). The results of the survey showed that many patients do not maintain the necessary level of oral hygiene, rarely visit the dentist and are dissatisfied with their oral health.

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Relevance. This study is related to the study of the etiology, pathogenesis and clinic of inflammatory bowel diseases (IBD). In a number of patients with IBD who are refractory to anti-inflammatory/immunosuppressive therapy, there are signs of current or transferred yersiniosis infection, not diagnosed by routine methods.Aim. According to the scientific literature and the results of our own research, to identify the presence and assess the nature of the influence of yersinia on the occurrence and development of IBD.Materials Methods. In our study, we conducted a bacteriological examination of feces by polymerase chain reaction (PCR) in patients with a diagnosis of IBD (UC and CD) in the acute phase (MMDAI ≥ 4 points for UC and Best index ≥ 150 points for CD), as well as immunological diagnostics using enzyme immunoassay (IFA) in the version of line-blotting (IB). Using the results obtained, clinical and instrumental semiotics were evaluated in subgroups of individuals suffering from IBD with confirmed yersiniosis ("IBD+ IER+") and without it ("IBC+ IER–"). We also assessed the role of intestinal infection by determining the effectiveness of initial antibacterial therapy in the treatment of exacerbation of IBD.Results. A significant part (in the case of our sample – 45%) of patients with IBD, when examined by high-tech methods, demonstrate the presence of markers of a transferred or current yersiniosis infection. Its role in the initiation and development of autoimmune intestinal inflammatory process requires further clarification.Conclusions. In 45% of patients with IBD, high-precision examination reveals markers of transferred or current yersiniosis infection. Routine clinical methods for the diagnosis of yersiniosis have low sensitivity and do not allow us to convincingly assess the role of this bacterial agent as a trigger factor for the onset or recurrence of IBD. There is a tendency for greater manifestation in the debut of IBD in patients with markers of systemic yersiniosis. The fact of improvement against the background of empirical antibacterial therapy in 24% of patients with IBD, achievement of early clinical remission in 57% of patients receiving antibiotic therapy, indicates a significant role of bacterial enterocolopathogens in the genesis of chronic intestinal inflammation in a number of patients.

Introduction. Today the search and development of new methods for diagnosing organic or functional pathology of the thyroid gland with an asymptomatic or clinically nonspecific course is an urgent task of endocrinology and therapy. The functioning of the thyroid gland and the state of the human intestinal microbiome are dynamically interrelated. One of the considered promising methods is mass spectrometry of microbial markers of the human intestinal microbiota.The purpose of the study. To study the microbial spectrum and characteristics of the biotic environment of the intestine in patients with thyroid diseases.Materials and methods. 21 patients were examined (8 men and 13 women, median age 40.5 [31.75; 54] years, respectively; 15 with thyroid disease, 6 without thyroid disease). In addition to the standard clinical and laboratory-instrumental examination, patients, colon contents were examined by chromatography-mass spectrometry of microbial materials. The work used descriptive non-parametric statistics with subsequent interpretation. Mean values of indicators and their variance are presented as median, upper and lower quartiles. In relation to representatives of the fecal microbiota, in addition to absolute values, the frequency of occurrence of the microorganism within the reference range, moderate or pronounced deviations was taken into account. The determination of the statistical significance of differences in the relative frequency value was carried out using Fisher’s exact test. The presentation of the results is implemented in the form of range diagrams, compactly depicting a one-dimensional probability distribution, tables. Results. Differences in the composition of the microbiota of the control group and the experimental group for Alcaligenes spp., Staphylococcus spp., Megamonas hypermegale, Peptostreptococcus anaerobius were revealed.Conclusions. The development of thyroid gland pathology is accompanied by significant deviations in the composition of the fecal microbiota, determined by mass spectrometry of microbial markers. In persons with thyroid pathology, in the absence of clinically significant external influences on the intestinal microbiome, a pronounced decrease in the level of Alcaligenes spp., Staphylococcus spp., is detected, a tendency to a decrease in the level of Megamonas hypermegale, Peptostreptococcus anaerobius is determined. Further differentiated study of the composition of the intestinal microbiome in patients with thyroid diseases is required, depending on the nosological affiliation and the nature of the endocrine dysfunction.

BACKGROUND: Non-invasive methods for diagnosing thyroid pathology with an inapparent, asymptomatic or clinically non-specific course is an urgent task of thyroidology today. It seems promising for such diagnostics to use a combination of mass spectrometry of microbial markers of the human intestinal microbiota with an assessment of the quality of life using the 36-Item Short Form Survey (SF-36) questionnaire. AIM: to study the features of intestinal microbiocenosis and subjective assessment of the quality of life in patients with thyroid diseases. MATERIALS AND METHODS: 21 patients were examined (8 men and 13 women, median age 40,5 [31,75; 54] years, respectively; 15 with thyroid disease of varying degrees of clinical manifestations, 6 without thyroid disease). In addition to the standard clinical and laboratory-instrumental examination, the patients underwent a study of the contents of the large intestine by the method of chromato-mass spectrometry of microbial markers. Through the use of the SF-36 methodology, an assessment of the quality of life was implemented. Descriptive, nonparametric statistics were used in the work, differences in relative frequency values were determined using Fishers exact test. The presentation of the results was carried out using range diagrams, compactly depicting a one-dimensional probability distribution, tables. RESULTS: Differences in the composition of the microbiota of the control group and the study group for Alcaligenes spp., Staphylococcus spp. were revealed, a downward trend in the level of Megamonas hypermegale, Peptostreptococcus anaerobius in the main group is determined. A tendency revealing a decrease in the quality of life in the study group compared with the control in terms of social functioning, role emotional functioning was revealed. CONCLUSIONS: Pathology of the thyroid gland is accompanied by deviations in the composition of the fecal microbiota, determined by the method of mass spectrometry of microbial markers. Characteristic features of changes in the intestinal microbiome are a decrease in the level of Alcaligenes spp., Staphylococcus spp., a tendency to decrease in the titers of Megamonas hypermegale, Peptostreptococcus anaerobius. Violation of the endocrine function of the thyroid gland can cause a deterioration in the quality of life, which is manifested by a tendency to reduce social functioning, role-playing emotional functioning.