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Catheter-associated urinary tract infection is mainly related to biofilm on the catheter surface which provides the opportunity for colonization and attachment resulting complex structured biofilm formation makes development of antibiotic resistant organism creates management of CAUTI is more critical. Therefore, there is an alarming call for drug development against multidrug resistant organisms. Hence, our study attempted the antibacterial activity of methanolic extract of Achyranthes aspera against Staphylococcus aureus one of the prevalent organisms involved in CAUTI. The A. aspera antibacterial activity was determined against S. aureus and minimum inhibitory concentration of A. aspera against S. aureus calculated was as 3.6 mg/ml which needed to inhibit the growth of tested microbe. The colony forming ability of S. aureus was studied in the presence of A. aspera methanolic extract using crystal violet staining method and the colony formation was observed after the MIC level indicates colony formation increases when decreasing the concentration of A. aspera. To ascertain the effect of A. aspera on biofilm formation after treatment with various concentrations, the biofilm formation assay performed. The A. aspera can able to reduce the biofilm formation as 80 and 88% for 1X MIC and 2X MIC against S. aureus indicating the antibiofilm forming ability. To prevent the microbial colonization on catheter surface, the coating of catheter with methanolic extract of A. aspera was investigated for its antibacterial activity against S. aureus using in vitro bladder model. The clear zone formations around the catheter piece indicating the anti-adhesive property of A. aspera against S. aureus. Collectively, methanolic extract of A. aspera can pave the way for new antibacterial agent against S. aureus.