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Effective energy expenditure is critical for maintaining body weight (BW). However, underlying mechanisms contributing to increased BW remain unknown. We characterized the role of brain angiogenesis inhibitor-3 (BAI3/ADGRB3), an adhesion G-protein coupled receptor (aGPCR), in regulating BW. A CRISPR/Cas9 gene editing approach was utilized to generate a whole-body deletion of the BAI3 gene (BAI3−/−). In both BAI3−/− male and female mice, a significant reduction in BW was observed compared to BAI3+/+ control mice. Quantitative magnetic imaging analysis showed that lean and fat masses were reduced in male and female mice with BAI3 deficiency. Total activity, food intake, energy expenditure (EE), and respiratory exchange ratio (RER) were assessed in mice housed at room temperature using a Comprehensive Lab Animal Monitoring System (CLAMS). While no differences were observed in the activity between the two genotypes in male or female mice, energy expenditure was increased in both sexes with BAI3 deficiency. However, at thermoneutrality (30 °C), no differences in energy expenditure were observed between the two genotypes for either sex, suggesting a role for BAI3 in adaptive thermogenesis. Notably, in male BAI3−/− mice, food intake was reduced, and RER was increased, but these attributes remained unchanged in the female mice upon BAI3 loss. Gene expression analysis showed increased mRNA abundance of thermogenic genes Ucp1, Pgc1α, Prdm16, and Elov3 in brown adipose tissue (BAT). These outcomes suggest that adaptive thermogenesis due to enhanced BAT activity contributes to increased energy expenditure and reduced BW with BAI3 deficiency. Additionally, sex-dependent differences were observed in food intake and RER. These studies identify BAI3 as a novel regulator of BW that can be potentially targeted to improve whole-body energy expenditure.

In our investigation we estimated spreading of main IHD modifiable risk factors in patients with acute coronary syndrome, living on the territory of Tumen region, and there compliance to therapy. 69 patients under age of 70 were included (average age 63 AE 0,5, 60% were men). 50,7 % were smokers at admittance to the hospital, or a month before the hospitalization; 12,5% among them were women. 21,7% had diabetes melitus and almost half of them hadn't known about there diagnosis and had been taking no hypoglycemic therapy. Glycosilated hemoglobin determination showed that only 19,7% of patients with diabetes controlled there glycemia for the previous 3 months (HBA1< 6,5%), and 33,8% of theese patients had HBA1>8%. 47,8 % of all patients were impaired glucose tolerance. Among all patients with hypertension (84,2%) only 17,1% effectively controlled there blood pressure. Despite high popularity in the last years of hypolipidemic therapy, 84,2% of our patients had total cholesterol above recommended level, while 14% were very hypercholesterolic (> 8 mmol/l). Only 16,4% gained LDLP target rates. More than a half (52,3%) of included into investigation were centrally obese and had exessive body mass.Compliance to therapy of the patients whose diagnosis of IHD had already been established before inclusion into investigation was rather poor. 54,1% of patients were using antiaggregates and long-term nitrates. Less preferable were ACEI/ARB and beta-blockers (47,9% and 43,8% respectevely). Calcium channel blockers and diuretic were taking 23% of patients. And only 10 used statins. In 6,25% of cases acute coronary syndrome was the result of clopidogrel intake cessation within 12 months after stenting with drug-eluting stents. 8,7% of patients were taking no medical therapy, having known of there diagnosis and having acute coronary syndrome in history.Our results showed that prevalent risk factor in Tumen rigion is hypercholesterolemia, central obesity and glucose metabolism disorders. This says about necessity of hypolipidemic therapy compliance intencification and timely detection of early glucose metabolism disorders.Background: Control of hypertension (HT) in Polish general population is poor. However, achievement of treatment goal for hypertension is considered as a basis of secondary prevention of coronary heart disease (CAD).

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