The objective of this study was to develop and validate a method for simultaneous quantitative analysis of allopurinol and lesinurad in bulk drug and pharmaceutical formulations. An isocratic HPLC analysis method using a reverse phase Waters spherisorb ODS1 C18 column (250 mm × 4.6 mm, 5 μ) and a simple mobile phase without buffer was developed, optimized and fully validated. Analyses were carried out at a flow rate of 0.9 mL/min at 50 °C and monitored at 246 nm. This HPLC method exhibited good linearity, accuracy and selectivity. The recovery (accuracy) of both allopurinol and lesinurad from all matrices was greater than 98 %. The allopurinol and lesinurad peak detected in the samples of a forced degradation study and no interference of excepients or the degradation products formed during stress study. The method was rugged with good intra- and inter-day precision and sensitive. This stability indicating HPLC method was selective, accurate and precise for the simultaneous analysis of allopurinol and lesinurad in pharmaceutical formulations.

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Excess volumes and sound velocities for binary liquid mixtures of acetophenone with n-alcohols and ialcohols are measured at 303.15 K. The sound velocity and density data are used to calculate the isentropic compressibilities (k,) and deviations in isentropic compressibilities (AkJ. The values of V' are negative in all the systems except in the system, acetophenone + n-heptanol wherein the V L values are positive.Deviations in isentropic compressibilities are negative in all the systems over the entire range of composition. The data are used to explain the possible intermolecular interactions between unlike molecules. K E Y WORDS: Sound velocity. compressibility.

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