To investigate the effect of different food items on fatty acids profile of Perinereis nuntia worms, Polychaete worms mentioned above were collected from the tidal shores of Bandar Abbas . In this study three treatments included nutritional Commercial post larval shrimp, green algae (Entermorpha. sp.) and red algae (Gracillaria. sp) each with three replications dry was used to feed the worms. Feeding twice daily and 10% of the body weight of worm for two months was conducted. The results showed that different treatments in terms of food fatty acid profile there is a significant difference (P<0.05) . Most fatty acids (SFA) 35.88 ± 0.01, (MUFA) 43.82 ± 0.01 and (PUFA) 108.98 ± 0.01 (mg g -1 ) of polychaete worms were fed red algae. Results suggest that Perinereis nuntia fed red algae have a well-balanced nutritional profile for penaeid shrimp and fish broodstock.