S. 1990. Lipid biosynthesis in seeds of mustard {Brassica juncea) influenced by zinc and sulphur deficiency. -Physiol. Plant. 80: 102-108.In developing seeds of mustard {Brassica juncea L. cv. RLM198) the period between 20 and 30 days after fertilization (DAF) was identified as the period of active lipid biosynthesis, although dry matter continued to accumulate until maturity. The period of lipid synthesis was associated with a decrease in starch, soluble sugars and protein, thus, giving rise to precursors for the biosynthesis of lipids. Besides decreasing the dry matter content (on both % and seed basis), Zn and S deficiency caused a significant (F < 0.05) reduction in oil content. As compared to control, the decrease in oil content was 11, 12 and 18% at ,30 DAF and 4, 9 and 16% at maturity in Zn, S and (Zn+S) deficient treatments, respectively. Throughout the period of seed development, a significant decrease in starch anil protein with a slight accumulation of soluble sugars was observed due to deficiency of Zn or S. The rate of [l-'^Cj-acetate incorporation into total lipids, which was maximal at 30 DAF, also displayed a significant decrease due to the abovementioned mineral deficiencies. Addition of Zn or S in vitro, enhanced the lipid synthesis at all stages of seed development. Under Zn and S deficiency, the phospholipids increased from 10 to 30 DAF and then declined until maturity. However, the proportion of giycolipids and free fatty acids increased, with a corresponding decrease in total giycerides. Further, io deficiency treatments, there was an increase in 22:1 with a corresponding decrease in 18:1, 18:2 and 18:3 in developing and mature mustard seeds.