Bhaishajya Kala is an important principle in our classics, which is to be considered while treating a disease. During treatment, success can be achieved only when there is proper combination of Desha, Kala, Pramana, Satmya, Asatmya, Pathya and Apathya. [1] Among these seven, Kala is given second position which reflects importance of Kala in Chikitsa. The relation between Aushadha and Kala is well established in classics. Acharya Charaka states that “medicine administered at appropriate Kala is more efficacious than one given in inappropriate Kala. ”[2] To highlight its role in Chikitsa, there is a necessity to analyze this concept which is the need of the hour. With the intention of practically validating this concept “Janu Sandhigatavata” has been taken up for the study. Panchatikta Guggulu Ghrita which has been taken for the study is seen to have beneficial therapeutic effects on Sandhigatavata as evidently seen in the Phalashruti. [3] As Bhaishajya Kala is not specifically mentioned for any Vatavyadhi so also for Janu Sandhigatavata, this study intends to find the appropriate Bhaishajya Kala for the same. Among all the Bhaishajya Kalas, in the present study only two Kalas were taken up which were suitable for Janu Sandhigatavata. The clinical trial was carried out on 40 patients. They were divided into two groups Group A and Group B consisting of 20 patients each. The results were analyzed based on the signs and symptoms. Statistical analysis between Group A and Group B for the parameters Sandhi Shoola, Shotha, Prasaranaakunchanyo Vedana, ROM and VAS painscale found no significant changes in the group. Individually each Group had highly significant results. But in the observation parameter of Atopa there was a difference of value between the groups, Group A was higher than Group B.