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: Hydroxyapatite, which is naturally and synthetically available, is often used as a biomaterial because of its similarity to bone. In this study; Natural hydroxyapatite powder, synthesized from sheep bone, and synthetic hydroxyapatite were used as matrix. Hybrid bio-ceramic composites were obtained by adding 5 wt. % expanded perlite-TiO2-ZrO2-MgO-P2O5 to both matrixes. The bio-ceramic materials which were mixed with mechanical mixer for 30 minutes were pressed with hydraulic press under 25 MPa pressure and sintered at 9000C for 1 hour. Density, micro-hardness, X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis were performed to determine characteristics of the samples. As a result; it was identified that the micro-hardness of natural hydroxyapatite was higher. In addition, the increase in micro-hardness values of ZrO2-reinforced samples was higher than the TiO2-reinforced samples. Hydroxyapatite, calcium silicate, calcium phosphate structures were observed in XRD analysis. Micro-pores were observed in TiO2-reinforced samples while more dense structures were observed in ZrO2-reinforced samples.