The present investigation was conducted in the University Laboratory, RVSKVV, Gwalior, MP, India during the period of February, 2020 to March, 2021. Guava fruits were randomly collected and cleaned in tap water to remove surface dust and leaves before weighing, sorting. This study was limited to five guava varieties viz., Allahabad Safeda, Gwalior-27, Lucknow- 49, Sheopur local and Lalit. The maximum fruit weight (292.14 gm) and volume of fruit (296.4ml) was found in the Sheopur local varieties. The maximum acidity (1.02%) was found in Allahabad safeda and no. of seeds/100gm of guava fruit (183.79) was found under lalit. The maximum TSS: acid ratio (21.76) and vitamin-C (ascorbic acid, 230.44mg/100gm) was found under Gwalior-27.