The present study was carried out to evaluate the in vitro cytoprotective effects of Psidium guajava and their isolated quercetin fraction to reduce the CCl 4 (carbon tetrachloride) induced toxicity in HepG2 cell lines (Hepatocellular carcinoma G2). Silymarin was used as a standard drug to compare the protective effects of plant extracts in infected cell lines. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5diphenyl-tetrazolium bromide) assay, cell viability assay, leakage parameters [Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Lactate dehydrogenase (LDH)], lipid peroxidation and reduced glutathione (GSH) levels were used to find out the protection of human derived HepG2 cells against CCl 4-induced damage. The levels of cytotoxicity, viability and GSH were reduced. While the activities of AST, ALT, LDH and lipid peroxidation was increased in CCl 4-treated groups. The treatment of P. guajava and their isolated quercetin fractions (100, 200, 300 lg/mL) decreased the elevated levels of all these parameters. The results of the present study suggest that the ethanolic extract of P. guajava leaf and their isolated quercetin fractions can able to reduce the CCl 4-induced cytotoxicity in HepG2 cell lines.

No abstract

No abstract

Background: Oxidative stress plays an important role in chronic complications of diabetes, cancer, liver disorder etc. The free radicals such as superoxide anions, hydrogen peroxides are causing the oxidative stress and it involves the cellular damage. Evidences recommended that the natural medicines from plant sources are treated to overcome the oxidative stress complications. Objective: The aim of the present is to find the antioxidant activity of the ethanolic extract of Terminalia catappa leaves and Terminalia chebula fruits in the doxorubicin (DOX) induced toxicity rats. Methods: Oxidative stress is induced with a single dose of doxorubicin and then the animals were treated with a dose of various concentration of ethanolic extract of T. catappa leaves and T. chebula fruits (200, 300 mg/kg/b.w) for 21 days. After the treatment, Lipid peroxide (LPO), Reduced glutathione (GSH), vitamin C, vitamin E, Glutathione-s-transferase (GST), Glutathione Peroxidase (GPx), Superoxide dismutase (SOD), catalase levels are determined. Propranolol 25mg/kg is used as standard drug. Results: In the present study, after the treatment of doxorubicin the levels of SOD, CAT, GSH, GST, GPX, vitamin C, vitamin E levels are decreased and LPO level is increased. After the treatment of T. catappa leaves and T. chebula fruits the levels were returned to the normal level. Conclusion: The results proved that the ethanolic extract of T. catappa leaves and T. chebula fruits may protects the cells from oxidative stress induced by the doxorubicin induced toxicity rats.

No abstract