Multiple neurological disorders are characterized by the abnormal accumulation of protein aggregates and the progressive impairment of complex behaviors. Our Drosophila studies demonstrate that middle-aged wild-type flies (WT, ~4-weeks) exhibit a marked accumulation of neural aggregates that is commensurate with the decline of the autophagy pathway. However, enhancing autophagy via neuronal over-expression of Atg8a (Atg8a-OE) reduces the age-dependent accumulation of aggregates. Here we assess basal locomotor activity profiles for single- and group-housed male and female WT flies and observed that only modest behavioral changes occurred by 4-weeks of age, with the noted exception of group-housed male flies. Male flies in same-sex social groups exhibit a progressive increase in nighttime activity. Infrared videos show aged group-housed males (4-weeks) are engaged in extensive bouts of courtship during periods of darkness, which is partly repressed during lighted conditions. Together, these nighttime courtship behaviors were nearly absent in young WT flies and aged Atg8a-OE flies. Previous studies have indicated a regulatory role for olfaction in male courtship partner choice. Coincidently, the mRNA expression profiles of several olfactory genes decline with age in WT flies; however, they are maintained in age-matched Atg8a-OE flies. Together, these results suggest that middle-aged male flies develop impairments in olfaction, which could contribute to the dysregulation of courtship behaviors during dark time periods. Combined, our results demonstrate that as Drosophila age, they develop early behavior defects that are coordinate with protein aggregate accumulation in the nervous system. In addition, the nighttime activity behavior is preserved when neuronal autophagy is maintained (Atg8a-OE flies). Thus, environmental or genetic factors that modify autophagic capacity could have a positive impact on neuronal aging and complex behaviors.

Stand density affects not only structure and growth, but also the health of forests and, subsequently, the functions of forest ecosystems. Here, we integrated dendrochronology and repeated inventories for ponderosa pine research plots to determine whether long-term growth and mortality responded to climate trends and how varying stand density influenced the responses. The plots were established prior to 1975 on existing stands throughout northern California. Although annual temperature increased consistently for the last 65 years, ring-width indices produced by eliminating age and thinning effects failed to detect radial trend regardless of site quality. However, interannual variation for the indices was substantial, reflecting a strong influence of climate on tree growth. Plot-level basal area increments were significantly affected by tree mortality. Stand density index explained most variation of mortality. Lowering stand density enhanced remaining tree growth, reduced mortality, and increased stand resiliency to disturbances and climate change. Besides higher climate moisture indices or lower vapor pressure deficits, any treatments that improve tree vigor and reduce stress will have a similar effect to reducing stand density. Although neither biotic disturbances nor abiotic conditions can be controlled, forest managers can manage stand density appropriately to enhance resilience to climate change and disturbances.

With increasing temperatures and projected changes in moisture availability for the Mediterranean climate of northern California, empirical evidence of the long-term responses of forests to climate are important for managing these ecosystems. We can assess forest treatment strategies to improve climate resilience by examining past responses to climate for both managed and unmanaged plantations. Using an experimental, long-term density and shrub removal study of ponderosa pine (Pinus ponderosa Lawson & C. Lawson) on a poor-quality site with low water-holding capacity and high runoff of the North Coastal mountain range in California, we examined the relationships between radial growth and climate for these trees over a common interval of 1977–2011. Resistance indices, defined here as the ratio between current year radial growth and the performance of the four previous years, were correlated to climatic variables during the same years. We found that all treatments’ radial growth benefited from seasonal spring moisture availability during the current growing year. Conversely, high spring and early summer temperatures had detrimental effects on growth. High-density treatments with manzanita understories were sensitive to summer droughts while lower densities and treatments with full shrub removal were not. The explanatory power of the climate regression models was generally more consistent for the same shrub treatments across the four different densities. The resistance indices for the lower density and complete shrub removal treatment groups were less dependent on previous years’ climatic conditions. We conclude that, for ponderosa pine plantations with significant manzanita encroachment, understory removal and heavy thinning treatments increase subsequent growth for remaining trees and decrease sensitivity to climate.

Abstract:The objectives of this review paper are to provide an overview of issues related to forest health and forest entomology, explain existing methods for forest insect pest detection, and provide background information on a case study of emerald ash borer. Early detection of potentially invasive insect species is a key aspect of preventing these species from causing damage. Invasion management efforts are typically more feasible and efficient if they are applied as early as possible. Two proposed approaches for detection are highlighted and include dendroentomology and near infrared spectroscopy (NIR). Dendroentomology utilizes tree ring principles to identify the years of outbreak and the dynamics of past insect herbivory on trees. NIR has been successfully used for assessing various forest health concerns (primarily hyperspectral imaging) and decay in trees. Emerald ash borer (EAB) (Agrilus planipennis), is a non-native beetle responsible for widespread mortality of several North American ash species (Fraxinus sp.). Current non-destructive methods for early detection of EAB in specific trees are limited, which restricts the effectiveness of management efforts. Ongoing research efforts are focused on developing methods for early detection of emerald ash borer.