Click into place: Tachyplesin‐I (TP‐I) analogues in which both disulfide bridges (1) have been replaced with triazoles (2) represent structural mimetics of TP‐I that display similar or slightly improved antibacterial activity. Optimized structures were obtained by replacing the cysteine residues in TP‐I by azido‐ and alkyno‐functionalized amino acids.

Peptide adhesion on semiconductor surfaces is quantitatively investigated by atomic force microscopy. The selected peptides are shown to cluster at the surface, with the larger, higher, and softer clusters appearing on the surfaces with lower peptide adhesion. Average cluster diameters vary from 40 nm on GaAs (100) to 300 nm on Si (100). Direct adhesion of the peptides to the surface competes with forming molecular aggregates that offer an overall reduced surface contact.

The difference between site-specific and random immobilisation of the aldo/keto reductase AKR1A1 was explored. AKR1A1 was recombinantly expressed as a thioester by the intein strategy. The thioester was selectively modified with a biotin label by the expressed protein ligation method, and subsequent immobilisation on streptavidin templates was performed. Adsorption of wild-type AKR1A1 to streptavidin templates and of biotinylated AKR1A1 to uncoated templates was used to study randomly immobilised enzymes. Investigation of the kinetic parameters revealed remarkably improved activity for the site-specifically immobilised enzyme, which was comparable to that of the wild-type enzyme in solution and 60-300-fold greater than that of the randomly immobilized enzymes. Furthermore, the enzyme was surprisingly stable. No loss of activity was observed for over a week, and even after 50 days more than 35% of activity was maintained.

Triazol statt Disulfid: Analoga von Tachyplesin‐I (TP‐I), in denen beide Disulfidbrücken (in 1) gegen Triazoleinheiten (in 2) ausgetauscht wurden, imitieren die Struktur von TP‐I und verfügen über eine ähnliche oder etwas stärkere antibakterielle Aktivität. Optimale Strukturen resultieren, wenn die Cysteinreste in TP‐I durch Aminosäuren mit Azid‐ und Alkinfunktionen ersetzt werden.

Solvent‐assisted decal transfer lithography (DTL) enables the formation of well‐defined micro‐/nanostructures over a large area (∼4 in. wafer) by combining irreversible oxygen bonding and anisotropic swelling of poly(dimethoxylsiloxane) (PDMS). Such swelling‐induced stress gradient allows for cohesion failure of the skin layer upon removal of the stamp, leaving behind a highly uniform layer (∼100 nm).