This study investigated the effects of display type, student population, and environment on search time, accuracy, workload, change of critical fusion frequency (CFF), and subjective visual fatigue while performing a reading task. Twenty students participated in the experiment, where three types of display (liquid crystal display, electronic paper display, and plain paper), two student populations (junior high school students and college students), and two types of environment (a controlled laboratory and a carriage of a public transit system) were investigated. The results showed that the student populations were significantly different in terms of search time, accuracy, and subjective visual fatigue. The results also showed that the interaction between display type and student population was significant on workload. The reading performance of accuracy for participants in the laboratory was significantly better than that in the public transit system.