No abstract

A novel and simple fluorescence method for Hg 2 + recognition and detection has been developed based on T-Hg 2 + -T complex and exonuclease III (Exo III) assisted cyclic signal amplification. Graphene oxide (GO) and carbon nanotubes (CNTs) were used to quench the fluorescence FAM-labelled single strand DNA (FAM-ssDNA). The optimum conditions, including GO and CNTs concentrations, enzyme concentration and enzymatic hydrolysis time, were studied. The linear range, regression equation and metal ion selectivity were further studied and compared. The recovery rates of Hg 2 + in tap water and urine were between 93.83-103.5%.[a] Prof. Figure 8. The differences in fluorescence intensity between solutions containing different metal ions. The concentration of Hg 2 + is 500 nM and those of other metal ions are10 μM. (A: MWCNTs system; B: GO system). ChemistrySelectFull Papers