Northern forest trees are challenged to adapt to changing climate, including global warming and increasing tropospheric ozone (O(3)) concentrations. Both elevated O(3) and temperature can cause significant changes in volatile organic compound (VOC) emissions as well as in leaf anatomy that can be related to adaptation or increased stress tolerance, or are signs of damage. Impacts of moderately elevated O(3) (1.3x ambient) and temperature (ambient + 1 degrees C), alone and in combination, on VOC emissions and leaf structure of two genotypes (2.2 and 5.2) of European aspen (Populus tremula L.) were studied in an open-field experiment in summer 2007. The impact of O(3) on measured variables was minor, but elevated temperature significantly increased emissions of total monoterpenes and green leaf volatiles. Genotypic differences in the responses to warming treatment were also observed. alpha-Pinene emission, which has been suggested to protect plants from elevated temperature, increased from genotype 5.2 only. Isoprene emission from genotype 2.2 decreased, whereas genotype 5.2 was able to retain high isoprene emission level also under elevated temperature. Elevated temperature also caused formation of thinner leaves, which was related to thinning of epidermis, palisade and spongy layers as well as reduced area of palisade cells. We consider aspen genotype 5.2 to have better potential for adaptation to increasing temperature because of thicker photosynthetic active palisade layer and higher isoprene and alpha-pinene emission levels compared to genotype 2.2. Our results show that even a moderate elevation in temperature is efficient enough to cause notable changes in VOC emissions and leaf structure of these aspen genotypes, possibly indicating the effort of the saplings to adapt to changing climate.
To study the effects of slightly elevated temperature and ozone (O3) on leaf structural characteristics of silver birch (Betula pendula Roth), saplings of four clonal genotypes of this species were exposed to elevated temperature (ambient air temperature +0.8–1.0 °C) and elevated O3 (1.3–1.4× ambient O3), alone and in combination, in an open-air exposure field over two growing seasons (2007 and 2008). So far, the impacts of moderate elevation of temperature or the combination of elevated temperature and O3 on leaf structure of silver birch have not been intensively studied, thus showing the urgent need for this type of studies. Elevated temperature significantly increased leaf size, reduced non-glandular trichome density, decreased epidermis thickness and increased plastoglobuli size in birch leaves during one or both growing seasons. During the second growing season, O3 elevation reduced leaf size, increased palisade layer thickness and decreased the number of plastoglobuli in spongy cells. Certain leaf structural changes observed under a single treatment of elevated temperature or O3, such as increase in the amount of chloroplasts or vacuole, were no longer detected at the combined treatment. Leaf structural responses to O3 and rising temperature may also depend on timing of the exposure during the plant and leaf development as indicated by the distinct changes in leaf structure along the experiment. Genotype-dependent cellular responses to the treatments were detected particularly in the palisade cells. Overall, this study showed that even a slight but realistic elevation in ambient temperature can notably modify leaf structure of silver birch saplings. Leaf structure, in turn, influences leaf function, thus potentially affecting acclimation capacity under changing climate.
To study the possible differences in tropospheric ozone (O3) tolerance of oat (Avena sativa L.) and wheat (Triticum aestivum L.), two oat and two wheat cultivars were exposed to 0, 50, or 100 nL·L–1 O3 concentrations in growth chambers. Measurements on volatile organic counpound emission and physiological, biochemical, and leaf structural characteristics were conducted with 2- and 4-week-old seedlings. Neither of the studied species was particularly O3 sensitive, but O3 sensitivity should rather be defined on the basis of the characteristics of the cultivars within species. Visible leaf injuries increased with leaf age and with increasing O3 concentration. Net photosynthesis (Pn), stomatal conductance (gs), and chlorophyll fluorescence (Fv/Fm) of 2-week-old seedlings were more detrimentally affected by O3 compared with older seedlings. Wheat generally invested more in photosynthesis and related processes, such as gs, Fv/Fm, concentrations of Rubisco, chlorophylls and carotenoids, and synthesis of starch compared with oat. O3 increased Rubisco concentration in 2-week-old and carotenoid concentration in 4-week-old seedlings, especially in wheat. Lower extent of O3-caused visible leaf injuries in the other oat cultivar can supposedly be explained by its low stomatal conductance and high monoterpene production.
At the University of Eastern Finland (UEF) Library, the national Open Science and Research initiative (2014–2017, Ministry of Education and Culture of Finland) triggered the planning and construction of open science related research support services. Planning of support services with themes of open access scholarly publishing, open research data and open study material began at full throttle at the UEF Library in November 2015. Information specialists were grouped into teams, which orientated to separate aspects of open science and shared their knowledge by training the whole library staff. Teamwork continued actively over the year 2016. Open science continuously brings new tasks for the Library and has already notably changed the job profiles of the library specialists.Advancing open science has been considered highly important not only at the library but also at the university level. UEF has offered resources e.g. by recruiting new information specialists and a data protection officer and internal auditor. UEF Library has a vital role in conducting open science but it is practiced in close collaboration with University Services, especially that of Development Services, General Administration and Legal Services and IT Services. Open Science team has landed the departments to share information and to discuss about open science practices at UEF. Nowadays these roadshows concerning UEF publishing and data policy, open access (OA) publishing as well as research data management and sharing are our focal operation.Work continues but the results can already be seen: In the OA ranking of research organisations in Finland (Ministry of Education and Culture of Finland), UEF has achieved level four in the five-level maturity model. Also, UEF researchers can order tailored training sessions about open research and support services from the diverse training menu offered by the Library. Updated Open UEF web pages are available for everyone and multi-channeled informing directed to UEF staff and students continues online. One concrete output from conducted open science and active campaigning about self-archiving is UEF institutional publication repository, UEF//eRepository, which was launched in February 2017. At the moment about 31% of UEF scientific publications are open access, but substantial increase is expected. The next big challenge in open research is data management and opening. UEF Library is starting to build a metadata portal for research data in order to conceive the data produced by UEF researchers and to help finding data for potential reuse.Open science will also be included in information retrieval studies of master's degree and doctoral students. To encourage students and teachers in OA publishing, during the international open access week, one student publishing master's thesis openly will be rewarded with a stipend by the Library. Department having the highest rate of OA master's theses will also be adorned with flowers.Open science is a matter of teamwork, committing and keeping up to date.
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