A novel bacterium, designated U33(T), was isolated from a soil sample collected in Mykhailyky, Poltavs'ka oblast, Ukraine. The bacterium was aerobic, Gram-positive, spore-forming, and consists of motile rods. The taxonomic position of strain U33(T) was studied by a polyphasic approach, and the results clearly showed that the phenotypic and chemotaxonomic properties are consistent with those of the genus Aneurinibacillus. The phylogenic analysis with 16S rRNA gene sequence of strains U33(T) showed the highest sequence similarity to those of Aneurinibacillus aneuriniticus ATCC 12856(T) (96.7 %), Aneurinibacillus migulanus DSM 2895(T) (96.7 %), Aneurinibacillus danicus NCIMB 13288(T) (95.8 %), and lower sequence similarity with other members of the genus Aneurinibacillus. Growth was observed at 20-55 °C (optimum, 37 °C) at pH 5.0-9.0 (optimum, pH 7) and with 0-5 % (w/v) NaCl (optimum, 2 % NaCl). The predominant menaquinone was MK-7 and the cell wall peptidoglycan consist of meso-diaminopimelic acid. The major cellular fatty acids are iso-C15:0 (58.0 %) and anteiso-C15:0 (13.2 %). The DNA G+C content of the strain U33(T) was 45.8 %. The physiological and chemotaxonomic characteristics distinguish strain U33(T) from the validly published species of genus Aneurinibacillus, and therefore, we consider this strain to represent a novel species of the genus Aneurinibacillus. The name Aneurinibaciilus humi sp. nov. is proposed with strain U33(T) (= KEMC7305-119(T) = JCM19865(T)) as the type strain.

A Gram-negative, non-motile, non-spore-forming and aerobic bacterium, designated FJ12(T), was isolated from seawater, Geoje-si, South Korea. Strain FJ12(T) was catalase and oxidase positive. Growth of strain FJ12(T) was optimally observed at 30 °C and pH 6 and grew in the presence of 0-11 % (w/v) NaCl (optimum 2-3 % NaCl). The phylogenic analysis based on 16S rRNA gene sequence of strains FJ12(T) showed the highest sequence similarity to those of Doghicola eburneus KCTC 12735(T) (95.4 %), Aestuariihabitans beolgyonensis KTCT 32324(T) (95.3 %), Sulfitobacter pontiacus KCTC 32185(T) (94.9 %), Roseisalinus antarcticus DSM 11466(T) (94.8 %) and Loktanella soesokkaensis KCTC 32425(T) (94.7 %). The major polar lipids of FJ12(T) were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid and unidentified lipids. It contained Q-10 as the predominant ubiquinone, and major fatty acid is C18:1 ω7c (51.7 %), C16:0 (24.0 %). The DNA G+C content of the strain FJ12(T) was 60 mol%. Based on physiological and chemotaxonomic characteristics, strain FJ12(T) should be regarded as the type species of a novel genus in the family Rhodobacteraceae for which the name Paradonghicola geojensis sp. nov. is proposed. The type strain FJ12(T) (=KEMB 3001-336(T)=JCM 30384(T)).