In this present research, the antioxidant activity of the metabolites produced by the marine bacteria Virgibacillus sp. associated with the sponge Callyspongia diffusa was evaluated and the crude metabolites was also tested to find out the chemical compounds. Methods: In view of testing the antioxidant activity, DPPH radical scavenging activity, Hydroxyl radical scavenging activity, Superoxide radical scavenging activity, Nitric oxide radical scavenging activity,, Metal chelating, Ferric reducing antioxidant power were carried out invitro. Results: The DPPH radical scavenging activity was 46.56% at 2000 mg/ml and the IC 50 value was 857.49 mg/ ml, The hydroxyl radical scavenging activity was found significant (60.77% at 2 mg/ml) and the IC 50 value was found 471.07 mg/ml. Superoxide radical scavenging activity was 25.58% at 2000 mg/ml and the IC 50 was 1353.28 mg/ml, for nitric oxide, the radical scavenging activity was 25.58% at 2000 mg/ml concentration and in the due course the IC 50 value was 1353.28 mg/ml. The results of the chemical analysis showed the presence of chemical components such as alkaloids, terpenoids, reducing sugars and anthraquinones. Conclusion: This research revealed the potential of marine sponge-associated bacterial bioactive compounds in scavenging the free radicals invitro. Separation of individual compounds from the crude metabolites of Virgibacillus sp. associated with the marine sponge C. diffusa will be resulted in the development of a novel antioxidant molecule.

The goal of the present work is to fabricate selenium nanoparticles utilising Morinda citrifolia leaves extract via green approach. UV-Vis spectroscopy, FT-IR, and TEM were used to characterise the green nanoparticles. The size of generated SeNPs in Morinda citrifolia was anticipated to be 12-160 nm based on TEM images. The antioxidant activity of selenium NPs was discovered to be 66.7 to 83.7% of free radical inhibtion. When the concentration of nanoparticles rises, the viability of cancer cells decreases. It shows that biosynthesized selenium nanoparticles have anticancer properties that depend on the concentration. The brine shrimp lethality assay revealed that Morinda citrifolia mediated selenium nanoparticles have low cytotoxic effects.

Several industries have become major contributors to waterbody contamination due to the improper removal of dyes and effluents into water bodies. Due to their carcinogenic properties and low biodegradability, dye degradation is a considerable danger to people, animals, and the oceanic environment. As part of this study, Andrographis paniculata leaf extract was used as a reducing and stabilizing agent to synthesize zinc nanoparticles and degrade dyes such as methyl red and eosin. Zinc oxide nanoparticles (ZnONPs) showed a surface plasmon resonance peak at 430 nm in the UV spectrum. The FTIR result showed a band at 597.93 cm−1 that confirmed the formation of zinc nanoparticles. AFM results revealed spherical ZnONPs. The SEM results predicted an average particle size of 60 nm for crystalline particles. Biologically synthesized zinc nanoparticles exhibited greater antibacterial activity against Pseudomonas spp. and Proteus spp. but lesser activity against Klebsiella spp. and S. aureus. At 1000 μg/ml concentration, ZnONPs had the highest antioxidant activity of 45.34%. An ultraviolet-visible spectrophotometer measured dye degradation progress between 300 and 800 nm. For methyl red, the maximum absorption peak was measured at 415 nm, and for eosin, the maximum peak value was measured between 500 and 515 nm.

Recently, nontoxic origin-mediated synthesis of copper oxide nanoparticles acquires further recognition because of the key role of bioapplications. The plant Cissus quadrangularis is one most prominent herbs used in the treatment of diabetes, asthma, tissue regeneration, etc. In this study, we tested the process of copper oxide nanoparticle synthesis and their role in many functions from Cissus quadrangularis. The synthesis of copper oxide nanoparticles uses plant extract and characterization by X-ray diffraction, thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FT-IR), atomic force microscope (AFM), and scanning electron microscope (SEM). The synthesized nanoparticles were analyzed for their biomedical applications such as antibacterial, antifungal, antioxidant, antidiabetic, and anti-inflammatory activity and antiproteinase action. The results show that the C. quadrangularis plant-mediated nanoparticles may be used in many biomedical applications related to arthritis, diabetes, and the production of various antimicrobial products in the future.

Background. Because of its diverse range of use in several ethics of diagnosis and care of multiple diseases, nanotechnology has seen remarkable growth and has become a key component of medical sciences. In recent years, there has been rapid advancement in medicine and biomaterials. Nanomedicine aids in illness prevention, diagnosis, monitoring, and treatment. Aim. The purpose of this work is to evaluate the antibacterial, anti-inflammatory, and cytotoxic capabilities of green produced silver nanoparticle with the addition of curcumin-assisted chitosan nanocomposite (SCCN) against wound pathogenic as reducing agents. Materials and Methods. The plant extract of Pongamia pinnata, silver nanoparticles, and its based curcumin nanoformulations was studied in this study utilizing UV visible spectrophotometer, selected area electron diffraction (SAED), and TEM. Anti-inflammatory, antimicrobial, and cytotoxic tests were performed on silver nanoparticles with the addition of curcumin-assisted chitosan nanocomposite (SCCN). Furthermore, these produced nanocomposites were coated on clinical silk and tested for antibacterial activity. Results. The produced silver nanoparticle with the addition of curcumin-assisted chitosan nanocomposite (SCCN) has significant antibacterial activities against Pseudomonas aeruginosa and staphylococcus aureus. They are as well as possess anti-inflammatory activity and furthermore prove to be biocompatible. Conclusion. This advancement in the field of biomaterials, which means nanocomposite, not only helps to reduce the harmful effects of pathogenic organisms while representing an environmentally benign material but it also shows to be a material with zero danger to humans and the environment.