Rhamnolipids mainly are produced by Pseudomonas aeruginosa and another microorganism that have been found to have good surface activity. Rhamnolipids are used in various application areas, including environmental, health, food, cosmetic, oil industries, pharmaceuticals and environmental bio remediation particularly in enhanced oil recovery (EOR) and cleaning of oil spills. Many kinds of bio surfactant such as, rhamnolipids are already being used in industry but it is important to develop indigenous technology for the production of rhamnolipids produced by the microorganisms of local origin which would be more suitable for the application to that specific areas. Rhamnolipids have several beneficial uses: they are easily degradable, nontoxic, nonmutagenic, and have the highest surface-tension-reduction index of any surface-tension reducing agent currently in use. In this review, I summarize the definition, preparation, properties, and the application in different areas especially in food and agriculture, and industrial potential of rhamnolipids, as the next generation of biosurfactants.