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Highlights The psychological stress and past trauma of refugees often force them to misunderstand the severity of COVID-19. The attempts of health care providers and aid agencies in the Rohingya refugee camps are limited to follow international/national guidelines to protect against the COVID-19 outbreak. The roles of local mediators to integrate the healthcare policies and the voices of the refugees are crucial to tackling COVID-19.

Pectinase is one of the important enzymes of industrial sectors. Presently, most of the pectinases are of plant origin but there are only a few reports on bacterial pectinases. The aim of the present study was to isolate a novel and potential pectinase producing bacterium as well as optimization of its various parameters for maximum enzyme production. A total of forty bacterial isolates were isolated from vegetable dump waste soil using standard plate count methods. Primary screening was done by hydrolysis of pectin. Pectinase activity was determined by measuring the increase in reducing sugar formed by the enzymatic hydrolysis of pectin. Among the bacterial isolates, the isolate K6 exhibited higher pectinase activity in broth medium and was selected for further studies. The selected bacterial isolate K6 was identified as Chryseobacterium indologenes strain SD. The isolate was found to produce maximum pectinase at 37°C with pH 7.5 upon incubation for 72 hours, while cultured in production medium containing citrus pectin and yeast extract as C and N sources, respectively. During enzyme-substrate reaction phase, the enzyme exhibited its best activity at pH of 8.0 and temperature of 40°C using citrus pectin as substrate. The pectinase of the isolate showed potentiality on different types of fruit juice clarification.

A pot experiment was executed to investigate the impact of biochar and compost with water-saving irrigation on the rice yield, water use efficiency, and physicochemical properties of soil. Two irrigation regimes—namely alternate wetting and drying (AWD) and continuous flooding (CF)—and four types of organic amendments (OA)—namely rice husk biochar (RHB), oil palm empty fruit bunch biochar (EFBB), compost and a control—were applied to evaluate their effects. Under the AWD irrigation regime, the maximum grain was produced by RHB (241.12 g), whereas under the same organic amendments, both AWD and CF produced a similar grain yield. Under the same organic amendment, a significantly higher water use efficiency (WUE) was observed from the AWD irrigation with RHB (6.30 g L−1) and EFBB (5.80 g L−1). Within the same irrigation regime, soil pH, cation exchange capacity, total carbon, total nitrogen and available phosphorus were enhanced due to the incorporation of biochar and compost, while higher soil exchangeable potassium was observed under CF irrigation for all treatments. RHB and EFBB significantly reduced the soil bulk density (up to 20.70%) and increased porosity (up to 16.70%) under both irrigation regimes. The results imply that the use of biochar with AWD irrigation could enhance the nutrient uptake and physicochemical properties of soil and allow rice to produce a greater yield with less water consumption.

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