In the present study, the green synthesis of silver nanoparticles (AgNPs) using ethanol extract of Cymodocea serrulata and biological activity were investigated by UV‐visible spectrophotometer, Fourier‐transform infrared spectroscopy (FTIR), x‐ray powder diffraction (XRD), and scanning electron microscopy. The results show that nanoparticles synthesized were confirmed by color change from green to dark brown. The XRD analysis confirmed that the AgNPs were crystalline and found that their UV maximum specific absorbance was between 200 and 400 nm, and their field emission scanning electron microscopy size was between 60 and 69 nm. FTIR studies identified different functional groups involved in the potential capping of AgNPs. The antidiabetic activity of the AgNPs was tested by the inhibition of carbohydrate digestive enzymes (a‐glucosidase and amylase). In addition, it has exhibited potential anticancer activity against breast cancer cells (MDF7). Hence, the present result warrants ecofriendly and efficient method in the synthesis of AgNPs, which can act as an alternative biomaterial for biomedical applications.

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