The microstructural evolution of an Al-10 wt.% Cu alloy was investigated during solidification at constant cooling rate by in situ synchrotron X-ray microtomography with a resolution of 2.8 lm. Solidification of this alloy leads to a coarse dendritic microstructure which was fully characterized in terms of variation with temperature of the solid fraction, the specific surface area of the solid-liquid interface and the local curvatures of the solid phase. By analysing the evolution with solid fraction of individual dendrites, at least two coarsening mechanisms were clearly identified in addition to solidification growth. The first mechanism involves remelting of small secondary dendrite arms to the benefit of bigger adjacent arms. The second is the coalescence of adjacent secondary arms, with progressive filling of the inter-arm spacing and coalescence at the tips. Although this mechanism preferentially occurs at high solid fractions, these results show that the evolution of the dendritic microstructure during solidification is complex and involves the occurrence of various mechanisms operating concurrently. In situ X-ray tomography thus allows revisiting the various models which have been proposed to account for dendrite coarsening during solidification.

ObjectiveTo explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they are attributable to an endochondral growth defect.MethodsKnee joints from STR/Ort mice with advanced OA and age‐matched CBA (control) mice were examined by Affymetrix microarray profiling, multiplex polymerase chain reaction (PCR) analysis, and immunohistochemical labeling of endochondral markers, including sclerostin and MEPE. The endochondral phenotype of STR/Ort mice was analyzed by histologic examination, micro–computed tomography, and ex vivo organ culture. A novel protocol for quantifying bony bridges across the murine epiphysis (growth plate fusion) using synchrotron x‐ray computed microtomography was developed and applied.ResultsMeta‐analysis of transcription profiles showed significant elevation in functions linked with endochondral ossification in STR/Ort mice (compared to CBA mice; P < 0.05). Consistent with this, immunolabeling revealed increased matrix metalloproteinase 13 (MMP‐13) and type X collagen expression in STR/Ort mouse joints, and multiplex quantitative reverse transcriptase–PCR showed differential expression of known mineralization regulators, suggesting an inherent chondrocyte defect. Support for the notion of an endochondral defect included accelerated growth, increased zone of growth plate proliferative chondrocytes (P < 0.05), and widespread type X collagen/MMP‐13 labeling beyond the expected hypertrophic zone distribution. OA development involved concomitant focal suppression of sclerostin/MEPE in STR/Ort mice. Our novel synchrotron radiation microtomography method showed increased numbers (P < 0.001) and mean areal growth plate bridge densities (P < 0.01) in young and aged STR/Ort mice compared to age‐matched CBA mice.ConclusionTaken together, our data support the notion of an inherent endochondral defect that is linked to growth dynamics and subject to regulation by the MEPE/sclerostin axis and may represent an underlying mechanism of pathologic ossification in OA.

Imaging techniques for quantifying how the hierarchical structure of deforming joints changes are constrained by destructive sample treatments, sample-size restrictions and lengthy scan times. Here, we report the use of fast, low-dose pink-beam synchrotron X-ray tomography combined with mechanical loading at nanometric precision for the in situ imaging, at resolutions lower than 100 nm, of mechanical strain in intact untreated joints under physiologically realistic conditions. We show that, in young, aged, and osteoarthritic mice, hierarchical changes in tissue structure and mechanical behaviour can be simultaneously visualized, and that tissue structure at the cellular level correlates with whole-joint mechanical performance. We also used the tomographic approach to study the co-localization of tissue strains to specific chondrocyte lacunar organizations within Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: