The purpose of the study was to determine whether the type of use, age and gender of Małopolski horses affect the level of selected haematological and biochemical parameters of their blood and whether there is an interaction between these factors. the research was carried out on 30 horses of the Małopolski breed, which were divided according to gender (18 mares and 12 stallions), age (2-6 years, 7-9 years and 10-16 years) and use (recreational vs. sport). To sum up, the gender of horses significantly affects erythrocyte indices, as evidenced by their significantly higher values in the stallions as compared to the mares, as well as by the interactions between gender and type of use and between the gender and age of the horse. The leukocyte and neutrophil counts increase with the age of horses, and the interaction between age and type of use indicates that the leukocyte and neutrophil counts in training horses is closely linked to their age. systematic physical exercise in sport horses increases leukocyte and lymphocyte counts and contributes to osteogenesis (increase in alp activity and plasma content of calcium and phosphorus), which has a beneficial effect on their health. The correlations obtained may improve breeders' awareness of the effect of various factors, such as age, gender or type of use, on blood indices in horses and can be helpful in evaluating the health of Małopolski horses. key words: horses, blood parameters, type of use, gender, ageIn recent years the growth of various forms of horse riding has been observed around urban agglomerations and in tourist areas. Interest in equestrian sport has also increased, leading to the emergence of many new equestrian disciplines. These factors have stimulated the development of equine exercise physiology (Neuberg and Geringer de Oedenberg, 2007). One of the most native and popular Polish breeds of *Work financed from statutory activity.
The aim of the study was to determine the effect of the inclusion of 2%, 4% and 6% extruded flaxseed in the diet of fattening pigs on their growth performance parameters, carcass parameters, and selected blood parameters. The experiment was conducted on 160 weaners (from about 30 kg to about 110 kg BW) assigned to four experimental groups of 40 animals each (5 replicates with 8 individuals each). The animals in control group received a diet in which the source of fat was soybean oil. The other groups received extruded flaxseed (FE) in place of soybean meal in the amount of 2% (group 2FE); 4% (group 4FE) and 6% (group 6FE). Soybean oil was added to the diets in the experimental treatments to obtain equal amounts of fat. The inclusion of 2% and 4% extruded flaxseed in the diet of fatteners in place of soybean meal significantly improved the digestibility of basic nutrients, i.e. ether extract (by about 5%) and dietary fibre (by more than 20%) and led to better growth performance (higher BW by about 11%; higher ADG by about 20% and lower FCR by about 12%) vs group C. In the groups with 2% and 4% extruded flaxseed there was also an increase in the lean meat content of the carcasses (by about 6%) and the thickness of the backfat (by about 6.5%) vs group C. The inclusion of extruded flaxseed did not affect the animals' condition, as indicated by the haematological and biochemical parameters of the blood, which were within reference ranges. The results for production, nutrient digestibility and carcass analysis between groups 2FE and 4FE were similar, and at this stage of research indicate that the use of 2% extruded flaxseed is economically more justified.
The aim of the study was to evaluate the effect of a diet containing extruded flaxseed (Linum usitatissimum L.) on the fatty acid composition of the loin, blood lipid parameters, and the redox status of tissues of finishing pigs. A total of 160 weaners (about 30–110 kg BW) were assigned to four experimental groups of 40 animals each (5 replicates with 8 individuals each). Group C (control) received a diet in which the fat source was soybean oil, while in groups 2FE, 4FE and 6FE soybean meal was replaced with extruded flaxseed in the amount of 2%, 4% or 6%, respectively. The diet containing extruded flaxseed reduced cholesterol levels in the blood plasma of pigs (grower 2FE and 6FE vs. C; finisher 2FE, 4FE and 6FE vs. C) and loin muscle (2FE, 4FE and 6FE vs. C). A decrease in the atherogenic LDL-C fraction and in the content of triacylglycerols was also noted in the blood plasma of grower and finisher pigs receiving flaxseed (2FE, 4FE and 6FE vs. C). The beneficial effects noted in the experimental pigs also included an increase in the overall content of n-3 PUFAs, especially ALA (18:3 n-3), and a reduction in the n-6/n-3 ratio. This was especially evident at 4% and 6% inclusion of flaxseed. The highest proportion of flax (6%) in the blend increased lipid peroxidation, as evidenced by the increase in the content of LOOH and MDA in the blood plasma of grower and finisher pigs. For this reason, a 4% share of flaxseed in the diet of fattening pigs seems to be optimal, while higher levels require an additional supply of exogenous antioxidants.
Thyme has strong antioxidant properties and, therefore, can reduce the intensity of oxidative processes taking place in the body. The study aimed to assess whether the addition of thyme to diets for fattening pigs containing extruded flaxseeds, a source of n-3 PUFAs, which are particularly susceptible to oxidation, would have a positive effect on redox status and lipid metabolism. The experiment was conducted using 120 weaners (WBP × Neckar crosses) of about 30 kg BW, which were kept until the end of fattening (about 110 kg BW) and divided into three groups of 40 pigs. The control group received a diet with 4% extruded flaxseed. In groups T1 and T3, 1% or 3% of thyme was added to the basal diet. The introduction of 3% thyme resulted in a decrease in the total cholesterol level in the blood and the loin muscle. Moreover, an increase in SOD and CAT activity and a decrease in FRAP and LOOH was noted. Following supplementation with 3% thyme, the n-3 PUFA content and n-3/n-6 ratio increased, while the SFA content was significantly reduced. The results of the studies indicate that thyme has a positive effect on the redox status and lipid profile of the blood and muscles.
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