A chronic toxicity study was conducted in earthworms (Pheretima peguana) exposed to soil spiked with chlorpyrifos at concentrations of 0, 0.1, 1, 10, and 100 mg/kg soil dry matter for 7, 14, and 28 days. The integrity of the coelomocyte lysosomal membrane, nervous system, and male reproductive tissue was monitored using, respectively, the neutral-red retention assay, acetylcholinesterase (AChE) enzyme assay, and histomorphology of spermatogenic cells in the seminal vesicles and cocoon production (at 28 days after 28 days' exposure). Chlorpyrifos decreased the coelomocyte neutral-red retention time (NRRT) significantly (p < 0.05) at concentrations > 0.1 mg/kg soil as early as day 7 of exposure and was dose- and time-dependent. Chlorpyrifos inhibition of AChE activity was greater at day 7 than at day14 (p < 0.05) indicating possibly nerve recovery. Chlorpyrifos induced concentration-dependent damage to spermatogenic cells and cytophores in premature stages. The number and size of premature, maturing, and fully mature spermatogenic stages were increased at low concentrations (<1 mg/kg) but a number of these maturation stages declined at higher concentrations (10 and100 mg/kg) on day 28. The most severe effects were observed in the maturing and fully mature stages at the highest chlorpyrifos concentration, and this had an adverse impact on cocoon production and cocoon viability. Collectively, the results suggest induction of widespread effects on multiple organ systems in P. peguana exposed to chlorpyrifos. Although NRRT and AChE activity were the most sensitive of the biomarkers, cocoon production and cocoon viability could still be considered as diagnostic tools for monitoring effects from low-dose long-term chlorpyrifos toxicity and for evaluating population effects. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1450-1459, 2016.

Stenomelania snails (Fisher 1885) have been reported from the coastal regions of the Indian Ocean and the Pacific Ocean, spanning India to Australia. Here, the species diversity and distribution of these snails in the south of Thailand are recorded. The snails were also examined for trematode infections in 13 locations in three Provinces, viz. Krabi, Trang and Satun, along the coast of the Andaman Sea. A total of 1,551 snails were in five morphs tentatively identified as Stenomelania aspirans, S. crenulata, S. punctata, S. torulosa and the closely-related Neoradina prasongi. With 10 infected snails, the trematode infection rate was 0.64%. The cercariae were categorised into three species from two morphologically-distinguishable types, viz. parapleurolophocercous cercariae (Haplorchis taichui and Procerovum cheni) and xiphidiocercariae (Loxogenoides bicolor), through the morphological characterisation of the larval stage. These trematodes were also analysed using the internal transcribed spacer subunit II region to confirm the species identity at generic and infrageneric levels.