Our findings suggest a trend towards inferior survival for regional cancer patients in WA compared with metropolitan-based patients.

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The ribonuclease T, (RNase T,) gene was ligated to a synthetic gene for the signal peptide of Escherichiu coli alkaline phosphatase. When this fusion gene was expressed in E. cofi under the control of the rrp promoter, active RNase T, having the correct N-terminal sequence was secreted into the periplasmic space, indicating that the heterologous signal peptide had been cleaved off correctly. The enzyme could be readily purified from the periplasmic fraction with a yield of 1.8 mg from 1 liter culture. Adopting the same strategy, it was possible to produce a labile mutant of RNase T, (Glu-58 + Ala mutant) in E. coli, the yield of the purified mutant enzyme being 2.0 mg from 1 liter culture.