Accurate and timely assessment of the severity of burn is essential for the treatment of burns. Currently, although most first-degree and thirddegree burns are easily diagnosed through visual inspection or auxiliary diagnostic methods, the seconddegree burn is still difficult to distinguish due to the ambiguity boundaries of second-degree with first-degree and third-degree burns. In this study, we proposed a non-invasive technique by combing photoacoustic imaging (PAI) and optical coherence tomography (OCT) to multi-parameter quantitatively assess the burns. The feasibility and capacity of the dualmode PAT/OCT for assessing the burns was first testified by tissuemimicking phantom and burn wounds in mouse pinna in vivo. The further