Color information plays an important role in the color image segmentation and real-time color sensor, which affects the result of video image segmentation and correct real-time temperature value. In this paper, a novel real-time color image segmentation method is proposed, which is based on color similarity in RGB color space. According to the color and luminance information in RGB color space, the dominant color is determined at first, and then color similarity can be calculated with the proposed calculation method of color component, which creates a color-class map. Next, the information of the corresponding color-class map is utilized to classify the pixels. Due to the characteristic that thermal inks feature color values that change in real time as the temperature changes, the segmentation results of thermal ink can be used as a real-time color sensor. Then, we also propose a method of color correction and light source compensation for the sake of potential inaccuracy of its measures. We discuss the proposed segmentation method application combining with color sensor (thermal ink) in real-time color image segmentation for Cyber physical system (CPS) by the application in fire detection and summarize a new method in identifying fire in a video based on these characteristics. The experiments showed that the proposed method in vision-based fire detection and identification in videos was effective; the results were accurate and can be used in real-time analysis.

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Milk beer is a new type of milk beverage produced by two-step fermentation of lactic acid bacteria and yeast and created first in China. Yeast is the key strain of milk beer fermentation, which determines the flavor and quality of milk beer. In this study, the preparation process of high-quality yeast strain MJ1 active freeze-dried powder suitable for milk beer fermentation was optimized. Results showed that the optimal freeze-dried protective agent was used: sucrose 11.27%, mannitol 4.88%, maltodextrin 15.43%, skimmed milk powder 7.82%, and applying the optimal freeze-drying conditions: rotation speed 6,000 rpm, centrifugation time 15 min, the mixing ratio of centrifugal bacterial sludge and the protective agent is 1:1.5, the mixing equilibrium time is 30 min, the cell survival rate of strain MJ1 can reach 90.21% ± 1.04%. It laid the foundation for the preparation of active freeze-dried yeast powder and the industrial production of milk beer. Novelty impact statement:(1) The formulation and conditions of the freeze-dried protectant of Kluyveromyces marxianus yeast strain MJ1 were optimized to improve the cell survival rate of the freeze-dried yeast powder, (2) the optimal storage conditions of the freeze-dried yeast powder of strain MJ1 were screened to provide theoretical reference for the long-term storage of freeze-dried yeast powder, and (3) the fermentation performance of the freeze-dried yeast powder of strain MJ1 was verified, which provided a reliable basis for the industrial production of milk beer.

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